a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%)
a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:2. For a fictional human tissue, it is hypothesised that cancer stem
cells have the following expression pattern of cell surface markers
(CD44+/CD24+/ESA+). Flow cytometry was used to isolate this
population of cells.
COMM
CD24
ESA
1%
Tumour volume (cm³)
a) In the flow cytometry experiment, explain how to determine
what percentage of the total cell population are the putative
cancer stem cells. Also, briefly describe two controls that
should be included in the experimental design. (30%)
3.01
2.5-
+602
In a follow-up experiment, sorted and unsorted cells were
injected subcutaneously into immunodeficient mice. The
following plot depicts the results from the experiment.
2.0-
1.5-
1.0-
CD24
0.5-
ESA
37%
• Unsorted
CD44+/CD24+/ESA+
CD44+/CD24-/ESA-
Time (weeks)
10 12
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