a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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2. For a fictional human tissue, it is hypothesised that cancer stem
cells have the following expression pattern of cell surface markers
(CD44+/CD24+/ESA+). Flow cytometry was used to isolate this
population of cells.
COMM
CD24
ESA
1%
Tumour volume (cm³)
a) In the flow cytometry experiment, explain how to determine
what percentage of the total cell population are the putative
cancer stem cells. Also, briefly describe two controls that
should be included in the experimental design. (30%)
3.01
2.5-
+602
In a follow-up experiment, sorted and unsorted cells were
injected subcutaneously into immunodeficient mice. The
following plot depicts the results from the experiment.
2.0-
1.5-
1.0-
CD24
0.5-
ESA
37%
• Unsorted
CD44+/CD24+/ESA+
CD44+/CD24-/ESA-
Time (weeks)
10 12
Transcribed Image Text:2. For a fictional human tissue, it is hypothesised that cancer stem cells have the following expression pattern of cell surface markers (CD44+/CD24+/ESA+). Flow cytometry was used to isolate this population of cells. COMM CD24 ESA 1% Tumour volume (cm³) a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%) 3.01 2.5- +602 In a follow-up experiment, sorted and unsorted cells were injected subcutaneously into immunodeficient mice. The following plot depicts the results from the experiment. 2.0- 1.5- 1.0- CD24 0.5- ESA 37% • Unsorted CD44+/CD24+/ESA+ CD44+/CD24-/ESA- Time (weeks) 10 12
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