Which tube from the three is the most appropriate to use and why?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin
C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated
from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified
mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains
purified mRNA isolated from pancreatic β-cells from a patient during absorptive state.

provide answers for the
following questions?
1) Which tube from the three is the most appropriate to use and why? 
2) How can you guarantee a high expression of your protein in any expression vector? 

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