The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents thepurines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation Wrepresents adenine or thymine.EnzymeTarget sequence|Cleavage5' GAATTC 3'|3' СТТААG 5'5'GAATTC 3'EcoRI|3' СТТААG 5'5' GATATC 3'|3' СТАТАG 5'5' GAT ATC 3'|3' СТА ТАG 5'EcoRV5' GGCC 3'3' CCGG 5'5' GG CC 3'|3' СС GG 5'HaellI5' AAGCTT 3'3' TTCGAA 5'5'A3' TTCGAAGCTT 3'HindIIIA 5'5' RGGWCCY 3'3' YCCWGGR 5'5' RG3' YCCWGGWCCY 3'PpuMIGR 5'Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substratestrand is omitted for clarity.5' ATCGAACTAGGCC 3'5' AAAGCTTGTGATATC 3'EcoRIЕcoRIEcoRVEcoRVHaellIHindIIIHindIIIHaellI

Restriction endonucleases are found in bacteria and they cut double-stranded DNA at the…

Answered: Which restriction endonucleases would…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut using three restriction enzymes, namely Kpnl, Sall and EcoRI, it yields four fragments with sizes of 390 bR. 810 bp, 270 bp www and 330 bp. Kpnl Sall EcoRI 390 810 270 330 1800 bp 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. 2. You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exac fragment sizes are not important.
The following are methods to control for bias during data collection, except: a. Assuring subjects' anonymity and confidentiality b. Reducing the number of participants c. Providing training for interviewers d. Randomly assigning subjects to experimental/control
Below is the vector pUST, gene W, and the restriction cut sites. What are 3 cutting strategies for cloning gene W into pUST
All of the following are true about Restriction endonucleases except fashion
Above are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment? Which enzyme, HindIII or EcoRI, produced a larger fragment? What part of DNA causes it to be negatively charged in order for electrophoresis to work?
Which of the following scenarios would ONLY occur if your skipped the digest purification step? UV/VIS spectrophotometric quantification of DNA may be skewed by uncut plasmid. Some plasmid molecules may be cut once, by one enzyme, and re-ligate to themselves. The fragments cleaved by the restriction enzymes on the plasmid and insert can re-ligate to their sites, causing reduced ligation efficiency. Plasmid molecules cut with EcoRI and Xbal can ligate to each other instead of the insert.
1) you have a locus in your PCR reaction that is a DNA squence is on the fifth chromosome, is a single copy locus, and has a squcne ID# 818. What is a name according to ISFG nomenclature 2) in electrophoresis system, DNA migartes toward____ which is _____ charged.
Consider the following: Restriction enzymes bind and cut between bases T and A. Based on this, which of the following are not acceptable VNTR segments? The DNA segment under consideration is AGTTAC GTTACA TCAATG CAATGT
After cloning, they transformed and plate bacterial cells using their cloned plasmid. Onto what type of growth medium will they plate their cells in order to distinguish between bacterial cells that obtained the plasmid and those that did not?
A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?
PCHEM4321. An agarose gel electrophoresis pattern of the plasmid PSPM4321 digestion (restriction) is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on the data given below. Hindlll Hindll BamHI +BamHI Figure 1: 1% agarose gel electrophoresis of pCHEM4321 40 24 16 12 12 8 4 4 + |
You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.

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Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' ATCGAACTAGGCC 3' 5' AAAGCTTGTGATATC 3' EcoRI EcoRI EcoRV EcoRV HaellII HindIII HindIII HaellI