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Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' ATCGAACTAGGCC 3' 5' AAAGCTTGTGATATC 3' EcoRI EcoRI EcoRV EcoRV HaellII HindIII HindIII HaellI

Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' ATCGAACTAGGCC 3' 5' AAAGCTTGTGATATC 3' EcoRI EcoRI EcoRV EcoRV HaellII HindIII HindIII HaellI

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme Target sequence |Cleavage 5' GAATTC 3' |3' СТТААG 5' 5'G AATTC 3' EcoRI |3' СТТАА G 5' 5' GATATC 3' |3' СТАТАG 5' 5' GAT ATC 3' |3' СТА ТАG 5' EcoRV 5' GGCC 3' 3' CCGG 5' 5' GG CC 3' |3' СС GG 5' HaellI 5' AAGCTT 3' 3' TTCGAA 5' 5'A 3' TTCGA AGCTT 3' HindIII A 5' 5' RGGWCCY 3' 3' YCCWGGR 5' 5' RG 3' YCCWG GWCCY 3' PpuMI GR 5' Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' ATCGAACTAGGCC 3' 5' AAAGCTTGTGATATC 3' EcoRI ЕcoRI EcoRV EcoRV HaellI HindIII HindIII HaellI
Transcribed Image Text:The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme Target sequence |Cleavage 5' GAATTC 3' |3' СТТААG 5' 5'G AATTC 3' EcoRI |3' СТТАА G 5' 5' GATATC 3' |3' СТАТАG 5' 5' GAT ATC 3' |3' СТА ТАG 5' EcoRV 5' GGCC 3' 3' CCGG 5' 5' GG CC 3' |3' СС GG 5' HaellI 5' AAGCTT 3' 3' TTCGAA 5' 5'A 3' TTCGA AGCTT 3' HindIII A 5' 5' RGGWCCY 3' 3' YCCWGGR 5' 5' RG 3' YCCWG GWCCY 3' PpuMI GR 5' Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' ATCGAACTAGGCC 3' 5' AAAGCTTGTGATATC 3' EcoRI ЕcoRI EcoRV EcoRV HaellI HindIII HindIII HaellI
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