1) The DNA fragment shown in Figure 1 is cleaved by the restriction enzyme EcoRI as indicated. The number in parenthesis shows the position of the cleavage site. The total length of the DNA fragment is 4000 bp. Small parts of the DNA sequence is known as shown. 5' ACCCTAGGTGTGACCGCGATCCGGCAGCATAAT 3' 01\/₁ EcoRI (400) EcoRI (1300) EcoRI (3800) 3' CGCGAAATGCTTTAAGCGCTCTACGGGAGGG_5' з'AGCGTTAGAGTAGCCGGTAAAGGGTACGCGCCTТАА 5' Figure 1: DNA fragment with a total length of 4000 bp. 6000 5000 4000 3000 2000 1000 800 600 500 400
1) The DNA fragment shown in Figure 1 is cleaved by the restriction enzyme EcoRI as indicated. The number in parenthesis shows the position of the cleavage site. The total length of the DNA fragment is 4000 bp. Small parts of the DNA sequence is known as shown. 5' ACCCTAGGTGTGACCGCGATCCGGCAGCATAAT 3' 01\/₁ EcoRI (400) EcoRI (1300) EcoRI (3800) 3' CGCGAAATGCTTTAAGCGCTCTACGGGAGGG_5' з'AGCGTTAGAGTAGCCGGTAAAGGGTACGCGCCTТАА 5' Figure 1: DNA fragment with a total length of 4000 bp. 6000 5000 4000 3000 2000 1000 800 600 500 400
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:1) The DNA fragment shown in Figure 1 is cleaved by the restriction enzyme EcoRI as indicated. The
number in parenthesis shows the position of the cleavage site. The total length of the DNA
fragment is 4000 bp. Small parts of the DNA sequence is known as shown.
5' ACCCTAGGTGTGACCGCGATCCGGCAGCATAAT 3'
EcoRI (400)
EcoRI (1300)
EcoRI (3800)
3' CGCGAAATGCTTTAAGCGCTCTACGGGAGGG5'
3'AGCGTTAGAGTAGCCGGTAAAGGGTACGCGCCTTAA 5'
Figure 1: DNA fragment with a total length of 4000 bp.
The figure below depicts a gel on which marker DNA of known size has been run. Sketch the location of
the bands that will appear, if the DNA fragment shown in Figure 1 is cleaved by EcoRI and afterwards run
on the gel along with the marker DNA.
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