Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample

Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter5: Phlebotomy Equipment

Section: Chapter Questions

Problem 5RQ

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A.Why do you plate the cells from the viable count on LB agar without ampicillin? B.If you observe 100 colonies on your 1/100 plate, how many colonies do you expect if everything works perfectly on your 1/1000 plate?
You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IRE
Comparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cells
The following is/are true about plate count EXCEPT a. A plate count is a count of viable or live cells b. Is based on the principle that viable cells replicate and give rise to visible colonies on plates incubated under optimum conditions c. Is based on the assumption that only one cell always lands on a particular spot in the plate and gives rise to one colony d. None of the above Clear my choice
Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?
A bacterial culture is initially composed of 100 cells. After 1 hour the number of bacteria is 1.5 times the initial population. a. If the rate of growth is proportional to the number of bacteria present, determine the time necessary for the number of bacteria to triple. b. What is the time required for a culture with 1x106 of the same bacteria to triple? Explain your results. c. Under what conditions would the answers obtained in (b) be invalid?
You collected 1000 mL of river water from the streamrunning through campus. You prepared dilutions of the water as follows:Tube A. 0.5 mL of river water + 99.5 mL of sterile waterTube B. 1 mL from Tube A + 9 mL of sterile waterTube C. 1 mL from Tube B + 9 mL of sterile waterTube D. 1 mL from Tube C + 9 mL of sterile waterYou then plated 0.1 mL of each dilution onto each of three MacConkey agarplates. (MacConkey agar is type of selective, differential medium. It inhibitsthe growth of Gram-positive microorganisms and non-enteric Gram-negative bacteria. Among the bacteria not inhibited by the medium,lactose-fermenting bacteria form pink colonies and bacteria that do notferment lactose form colorless colonies.) After incubation, the colonieswere too numerous to count on the plates prepared from dilutions A, B, andC. For the plates prepared from dilution D you counted the followingnumbers of colonies:Plate 1: 157 pink colonies and 62 colorless coloniesPlate 2: 167 pink colonies and 67…
As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 ml
At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
You collected 1000 mL of river water from the stream running through campus. You prepared dilutionsof the water as follows:Tube A. 0.5 mL of river water + 99.5 mL of sterile waterTube B. 1 mL from Tube A + 9 mL of sterile waterTube C. 1 mL from Tube B + 9 mL of sterile waterTube D. 1 mL from Tube C + 9 mL of sterile waterYou then plated 0.1 mL of each dilution onto each of three MacConkey agar plates. (MacConkey agar is type of selective,differential medium. It inhibits the growth of Gram-positive microorganisms and non-enteric Gram-negative bacteria.Among the bacteria not inhibited by the medium, lactose-fermenting bacteria form pink colonies and bacteria that do notferment lactose form colorless colonies.) After incubation, the colonies were too numerous to count on the platesprepared from dilutions A, B, and C. For the plates prepared from dilution D you counted the following numbers ofcolonies:Plate 1: 155 pink colonies and 50 colorless coloniesPlate 2: 165 pink colonies and 55…
a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.