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Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.

Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?

Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.
Transcribed Image Text:Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.
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