6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take.1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml Oc) 6.1 X 10² CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B O e) Plates A, B and C

6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take.1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml Oc) 6.1 X 10² CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B O e) Plates A, B and C

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______
Isolate #2: Cell Morphology (shape) . a. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? (1 pt) ***Please answer the four questions base on the picture provided.***
Jack, a new MLT at Cape Fear Hospital was given the following information from a patient's urine sample which had been tested for the presence of S. aureus. The urine contained 6.4 x 107 CFU/ml S. aureus, the plate dilution was 10, and the sample had been taken from the 10-5 dilution tube. Calculate the number of bacterial colonies which must have been counted on the plate and what volume of the sample in µl that was actually plated to give the above results. PS, both of these values can be calculated.
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7. E. Coli bacteria were grown in a 5.0 L batch cell culture reactor, with the following data collected: Cell Count (per mL) 10,277,000 Inoculation Time ---> Time 11:19 am 11:35 am 11:57 pm 12:08 pm 12:45 pm 1:07 pm 1:11 pm 1:16 pm 1:19 pm 1:34 pm 1:46 pm 1:57 pm 2:09 pm 2:33 pm 10,313,000 20,984,000 29,750,000 60,202,000 210,909,000 224,750,000 227,960,000 229,216,000 229,115,000 227,260,000 204,935,000 183,745,000 146,103,000 (inoculation is when the cells are first put in the growth media) A. Draw a log10 X vs. time graph and label the phases for batch cell growth. B. Calculate the specific growth rate, μ, and mass doubling time, td, for the exponential growth phase. C. Calculate kd, the specific rate of cell mass loss due to cell death (For B & C, it will be helpful to re-plot the y-axis as In X or In (X/X.), and focus on only the data in that phase) Using spreadsheet software will be helpful in drawing this graph and analyzing data for parts B & C. Be as quantitative as possible,…
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Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
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8. Tube number 1 contains a broth culture of bacteria. You transfer 30ml from tube 1 and place it into tube 2, which contains 270 ml of diluent. Calculate the dilution performed to create tube 2. What is the dilution factor between tubes 1 and 2? b. If the concentration of bacteria in tube 2 is 2x106 cells/ml, what is the concentration of a. bacteria in tube 1? с. If tube number 1 contains 5ml of bacterial culture, what is the total number of bacterial cells present in tube 1? d. What is the total number of bacterial cells in tube 2?
Hi, my question is 6 ml of urine is added to 30 ml of water. 0.1 ml is plated. 109 colonies form. What is the CFU/ml of the urine culture?
1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population ofthe culture, by transferring 25 mlof the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDI Sampling COUNTS 1st dilution 54; 61 TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 1st 2nd 3rd TNTC * TNTC = Too numerous to count i. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations. a. 1s* Sampling b. 2d Sampling c. 3* Sampling