Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 µl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C culture was then diluted 1/10 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.2: Disorders Of Hemostasis
Problem 11RQ
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what are the volume dilution and dilution factor for bacteriophage A,B,C
Experimental set up: all culture plates were incubated at 37°C over night
Bacteriophage A
200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to
10-4. 100 µl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish.
Bacteriophage B
100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10
to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Bacteriophage C
250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10
to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Transcribed Image Text:Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 µl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
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