Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 µl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C culture was then diluted 1/10 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 µl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C culture was then diluted 1/10 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
Question
what are the volume dilution and dilution factor for bacteriophage A，B，C
Transcribed Image Text:Experimental set up: all culture plates were incubated at 37°C over night
Bacteriophage A
200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to
10-4. 100 µl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish.
Bacteriophage B
100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10
to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
Bacteriophage C
250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10
to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.
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