9). E. coli strain B was coinfected with two phage strains: one having a large clear plaque (hr) phenotype and the other with a small turbid (h+r+) phenotype. Locus h regulates the host range and locus r regulates the rate of lysis. The progeny phages were collected and plated on a mixed culture of E coli B and B/2. Use the table below to answer the following questions. Plaque Morphology Large, clear Small, turbid Small, clear Large, turbid Frequency 615 593 95 84 Genotype a). Do you need the rule of 2 in this case? Why or why not? b). What are the genotypes of each progeny and which genotypes are parental vs. recombinant? c). Calculate the frequency of recombination (not map distance) between the h and r loci. Use two decimal places in your answer.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.


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