Which of the following characteristics of Taq polymerase makes it especially well suited for use in PCR reactions? OA It does not need a 3'OH in order to extend DNA strands. OB. It can recognize and amplify only specified gene sequences. OC. It can recognize and amplify only specified gene sequences. OD. It remains functional at high temperatures. Reset Selection

Which of the following characteristics of Taq polymerase makes it especially well suited for use in PCR reactions? OA It does not need a 3'OH in order to extend DNA strands. OB. It can recognize and amplify only specified gene sequences. OC. It can recognize and amplify only specified gene sequences. OD. It remains functional at high temperatures. Reset Selection

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 7QP: Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is...

See similar textbooks

Similar questions

PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased about a billion times. Why is the DNA polymerase from the bacteria Thermus.aquaticus used in PCR? Because it uses primers specific to sequences in a disease causing gene or organism to diagnosis diseases, etc. O It is used to flank the gene to be copied. O It allows scientists to be able to determine the variable number of tandem repeats of minisatellites or microsatellites to determine an individual's DNA fingerprint. O It is present and needed because the enzyme is resistant to very high temperatures since the bacteria live in hot springs. Due to the walls of the he plant cell, which are thick; therefore it is used to degrade the walls for PCR to begin.
Okazaki fragments are a direct consequence of: O The lagging strand being more highly methylated than the leading strand. O Ligase interfering with the DNA synthesis on the lagging strand. O A different kind of polymerase adding nucleotides on the lagging strand. O The way DNA unwinds. DNA polymerase only being able to add new nucleotides to a 3' end of a growing DNA strand.
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Part A Which of the following statements concerning restriction enzymes is true? Select all that apply. ►View Available Hint(s) ☐ Restriction enzymes specifically target and cut RNA in a sequence-specific manner. Restriction enzymes occur naturally in viruses as a defense mechanism against bacteria. Some restriction enzymes generate overhangs in the target DNA sequence upon incubation. During a cloning experiment, the vector and target DNA should be cut with different restriction enzymes to ensure that sticky ends are generated. Submit
Some Steps Involved in Creating Recombinant DNA 1. Plasmid DNA is cut at the restriction site.2. Foreign DNA is cut at the restriction site.3. Plasmid DNA is opened and sticky ends are formed.4. Foreign DNA restriction fragments are isolated5. Target sequence in the plasmid is recognized by the restriction endonuclease.6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.8. DNA ligase splices the foreign restriction fragment and the plasmid together. Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. Start with preparation of the plasmid DNA. Select one: a. 6, 2, 4, 7, 8, 5, 1, 3 b. 5, 1, 3, 6, 2, 4, 8, 7 c. 5, 1, 3, 6, 2, 4, 7, 8 d. 6, 2, 4, 5, 1, 3, 8, 7
Primers are used in both PCR and in DNA sequencing. What is the purpose of the primers? O to separate various-sized DNA products O to provide a 3'- OH for Taq polymerase to build a new DNA strand O to add nucleotides to a growing DNA strand O to separate the DNA template strands O to terminate synthesis of the growing DNA strand
Which of the following enzymes do not require a DNA template for nucleotide synthesis? * O DNA polymerase I RNA polymerase O Primase O poly-A polymerase O DNA polymerase II
Why is Taq polymerase used in a polymerase chain reaction (PCR)? O Unlike other DNA polymerases, Taq polymerase is heat stable and survives the 94 degree denaturation step in PCR. Taq polymerase is more efficient than other DNA polymerases. O Taq polymerase can produce DNA from an RNA template, unlike other DNA polymerases. O Taq polymerase has proofreading activity and thus makes fewer errors than other DNA polymerases. Question 44 Which of the following statements about the differences between prokaryotic and eukaryotic genomes is FALSE? O Prokaryotic genomes have centromeres, whereas eukaryotic genomes do not. O Eukaryotic genomes have telomeres, whereas prokaryotic genomes do not. Prokaryotic genomes have one origin of replication, whereas eukaryotic genomes have multiple origins of replication. O Prokaryotic genomes are less complex than eukaryotic genomes.
Part 3. Compare the Specificity of DNA-Cutting Tools The flexibility and specificity of CRISPR-Cas9 technology offer a large step forward for gene editing. The first DNA "scissors" were restriction enzymes, which cut DNA at predefined sequences, typically 4-8 base pairs long. For example, EcoRI, a restriction enzyme found in E. coli, will cut double- stranded DNA at every GAATTC sequence. If EcoRI were added to a sample that contained the entire human genome, it could cut at every GAATTC sequence. We can calculate the probability that a particular nucleotide sequence, such as GAATTC, will occur within a larger sequence. Table 1 below shows the calculated probabilities of finding sequences of particular lengths. These calculations are based on the assumption that DNA sequences are entirely random and that every nucleotide position has an equal probability of being A, T, C, or G. Use the table to answer the following questions. Table 1. Calculated probabilities of finding a specific…
You are characterizing a new DNA polymerase. In a test tube, you incubate the enzyme with all the components needed for catalysis, EXCEPT you don't add any dNTPs. However, you notice that some type of catalysis has occurred. What is the most likely product in this case? O The supercoils in the DNA have been relieved O The sugar has been converted from a deoxyribose to a ribose A DNA strand is one base shorter due to nuclease activity The magnesium ion has been converted to a charge of +2 to +1
A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP O 5'-ATTCGACT-3' O 5'-TCAGCTTA-3' What is the base sequence of the synthesized fragment? O 5'-AGTCGAAT-3' ddCTP O 5'-TAAGCTGA-3' I ddGTP
...... doesn't need during vitro transcription dna template s dNTPs rns polymerase all none