You are characterizing a new DNA polymerase. In a test tube, you incubate the enzyme with all thecomponents needed for catalysis, EXCEPT you don't add any dNTPs. However, you notice thatsome type of catalysis has occurred. What is the most likely product in this case?O The supercoils in the DNA have been relievedO The sugar has been converted from a deoxyribose to a riboseA DNA strand is one base shorter due to nuclease activityThe magnesium ion has been converted to a charge of +2 to +1

DNA is a molecule made up of two strands twisted around each other in a double helix shape. Each…

Answered: You are characterizing a new DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Pyrimidine dimers in DNA may be repaired by in E. coli cells. (select all correct answers) Select one or more: O a. photolyase O b. nucleotide excision repair O c. mis-match repair O d. methyl-transferase
What is the real definition of DNA ligase to make it true
Similar to prokaryotes, eukaryotes have a topoisomerase-ll-class enzyme called DNA gyrase, which can introduce negative supercoils into the relaxed circular DNA using energy of ATP. O True O False e Ques Moving to another question will save this response
Which of the following statement(s) correctly describe(s) telomerase? Select all that apply. You may select multiple options. ✔Telomerase synthesizes DNA from an RNA template. O All of these options O Telomerase extends the lengths of telomeres of linear chromosomes in eukaryotes. O Telomerase unwinds the DNA duplex at telomeres. Telomerase serves as a primer for DNA synthesis at telomeres. O Telomerase has 5'-to-3' exonuclease activity.
On paper, replicate the following segment of DNA: 5’ A T C G G C T A C G T T C A C 3’ 3’ T A G C C G A T G C A A G T G 5’a. Show the direction of replication of the new strands and explainwhat causes lagging and leading strands to show different patternsof replication.b. Explain how this is semiconservative replication. Are the newstrands identical to the original segment of DNA?
Which one of the following is true of DNA polymerase, based on our coverage? The 2'OH of the dNTP H-bonds to the palm region to position the dNTP near the primer. It has at least one metal ion that serves as a bridge' between the enzyme and the substrate. O The Mg2 is a strong nucleophile. It has a double-displacement mechanism. The attacker in the mechanism of action is considered to be the alpha-phosphate of the dNTP
What is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA. The Chelex denatures the DNA and makes the DNA single stranded. The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells. The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNA
Which of the following enzymes has 5' to 3' exonuclease activity? DNA polymerase lII O Telomerase DNA ligase O Primase DNA polymerase l
I'm a bit confused about this question will someone help me understand the concept
RNA synthesis in eukaryotes and prokaryotes occurs in the 3' to 5 direction. O none of the choices are correct. O is catalyzed by a DNA polymerase. O precedes DNA synthesis.
Reciprocal strand exchange is associated with ... O DNA photolyase site-specific recombination general recombination O DNA excision/repair
The function of gamma (y) complex of DNA polymerase III is to Select one: O a. to unwind double strand DNA (template DNA). O b. to associate the core DNA polymerase llI with template DNA. O c. to stimulate 5' to 3' exonuclease activity O d. to upload beta subunit of DNA polymerase III to template DNA.

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