Use the following available information answer questions 1-5. Protein name Molecular mass pl Subunit structure Protein A 250,000 9.7 Monomer Protein B 75,000 13,000 46,000 4.8 Tetramer Protein C 3.1 Monomer Protein D 10.2 Monomer 1. Name one purification method that can separate Protein A from a solution that contains all 4 proteins. 2. If you do ion-exchange chromatography at pH 7 on a column of diethylaminoethyl (DEAE)-cellulose, which of the above proteins will bind to the column? 3. If you do ion-exchange chromatography at pH 7 on a column of carboxymethyl (CM)-cellulose, which of the above proteins will not bind to the column? 4. If you do isoelectric focusing on a mixture of the above proteins followed by SDS-PAGE, and stain the gel with Coomassie Blue, how many protein spots do you expect to see? Explain your answer. 5. If I saw 5 spots in the experiment described in question 4, how will you explain the results?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
icon
Concept explainers
Question

Name one purification method that can separate Protein A from a solution that contains all four proteins. (Size exclusion chromatography, ion exchange chromatography, Purification by affinity chromatography, High pressure liquid chromatography, Isoelectric focusing, SDS-PAGE, or Two-dimensional Electrophoresis)

2. In picture

3. In picture

4. In picture

5. In picture

Use the following available information answer questions 1-5.
Protein name
Molecular mass
pl
Subunit structure
Protein A
250,000
9.7
Monomer
Protein B
75,000
13,000
46,000
4.8
Tetramer
Protein C
3.1
Monomer
Protein D
10.2
Monomer
1. Name one purification method that can separate Protein A from a solution that contains all 4 proteins.
2. If you do ion-exchange chromatography at pH 7 on a column of diethylaminoethyl (DEAE)-cellulose,
which of the above proteins will bind to the column?
3. If you do ion-exchange chromatography at pH 7 on a column of carboxymethyl (CM)-cellulose, which
of the above proteins will not bind to the column?
4. If you do isoelectric focusing on a mixture of the above proteins followed by SDS-PAGE, and stain the
gel with Coomassie Blue, how many protein spots do you expect to see? Explain your answer.
5. If I saw 5 spots in the experiment described in question 4, how will you explain the results?
Transcribed Image Text:Use the following available information answer questions 1-5. Protein name Molecular mass pl Subunit structure Protein A 250,000 9.7 Monomer Protein B 75,000 13,000 46,000 4.8 Tetramer Protein C 3.1 Monomer Protein D 10.2 Monomer 1. Name one purification method that can separate Protein A from a solution that contains all 4 proteins. 2. If you do ion-exchange chromatography at pH 7 on a column of diethylaminoethyl (DEAE)-cellulose, which of the above proteins will bind to the column? 3. If you do ion-exchange chromatography at pH 7 on a column of carboxymethyl (CM)-cellulose, which of the above proteins will not bind to the column? 4. If you do isoelectric focusing on a mixture of the above proteins followed by SDS-PAGE, and stain the gel with Coomassie Blue, how many protein spots do you expect to see? Explain your answer. 5. If I saw 5 spots in the experiment described in question 4, how will you explain the results?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Proteins
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON