A solution containing two globular proteins each with a single subunit (one is "large" with a radius of 47 A and the other is "small" with a radius of 16 A) is applied to a gel-filtration (ie. size-exclusion) column. What is the most likely outcome? O a The outcome will depend on which protein has the most negative surface charge. O b. The small protein elutes before the large protein. O. The large protein elutes before the small protein. O d. Both proteins elute together. O e. The outcome will depend on the pH of the solution.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
icon
Concept explainers
Question
100%
A solution containing two globular proteins each with a single subunit (one is "large" with a radius of
47 Å and the other is "small" with a radius of 16 A) is applied to a gel-filtration (i.e. size-exclusion)
column. What is the most likely outcome?
O a The outcome will depend on which protein has the most negative surface charge.
O b. The small protein elutes before the large protein.
O C. The large protein elutes before the small protein.
O d. Both proteins elute together.
O e. The outcome will depend on the pH of the solution.
Transcribed Image Text:A solution containing two globular proteins each with a single subunit (one is "large" with a radius of 47 Å and the other is "small" with a radius of 16 A) is applied to a gel-filtration (i.e. size-exclusion) column. What is the most likely outcome? O a The outcome will depend on which protein has the most negative surface charge. O b. The small protein elutes before the large protein. O C. The large protein elutes before the small protein. O d. Both proteins elute together. O e. The outcome will depend on the pH of the solution.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Macromolecules
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON