Two co-op students at your start-up company have been asked to evaluate the rate of reaction occurring in atransparent gel particle containing immobilized mouse melanoma cells. The equation for the reaction rateris is:VmaxT'AsKm+CAswhere R=3.2 mm, Vmax= 0.12 gmol s' m3, CAS = 41 gmol m3, and Km=0.8 gmol m3.One student reports a reaction rate of 1.6x10 gmol st; the other reports 1.6x10 1º gmol s1. You left yourcalculator on the bus this morning, but must know quickly which student is correct. Use an order-of-magnitude calculation to identify the right answer.Which student is correct?

An enzyme is a cellular catalyst that speeds up chemical reactions. Proteins that bind to…

Answered: Two co-op students at your start-up…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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How do you seal sequence 2 (from 5' end) to the 3' end of the sequence 1 in vitro condition? Design a splint oligomer and write the all-possible content(s) needed for reaction to occur. Sequence 1: 5'ACTGTCGATGCTAGCTTGATCCAAGTATTGCTAGACAGAATTGACATATAGGCGATGCTAGT3' Sequence 2: 5'ATCGCTAGGATCGCTAGATTTAAGTCGCTGATCGGCTAGATATAACAGGTCCTGAATCGCTA3'
Mutants of Neurospora crassa that lack carbamoyl phosphate synthetase I (CPS I) require arginine in the medium in order to grow, whereas mutants that lack carbamoyl-phosphate synthetase II (CPS II) require a pyrimidine, such as uracil. A priori, one would expect the active CPS II in the arginine mutants to provide sufficient carbamoyl phosphate for arginine synthesis, and the active CPS I in the pyrimidine mutants to “feed” the pyrimidine pathway. Explain these observations.
tcyDTDO is a reactive molecule. A. WHy might this cause a problem in terms of side effects? B. What is an experiemnt you could run on cells (no aminals of humans allowed!) to see if this is actually a problem? For A, I understand that tcyDTDO is a molecule that cleaves disulfide bonds to create unfolded proteins. This molecule is used to induce apoptosis in cancer cells. However, it also has the property of causing unfolded proteins, leading to induce cell death in normal cells as well. Howeer, I don't know part B of the question. Glad if the expert could advise.
In biochemical and structural terms, how does the cellular form of the prion protein differ from the infectious form? Can you also explain what is the biochemical basis for “infectiousness” of the prion protein?
Describe the concept of isoelectric point and calculate the PI for isoleucine?
If a particular cell type requires two passes through a Manton-Gaulin homogenizer to achieve a R of at least 0.90, what is the minimum number of passes required to achieve a disruption of at least 0.95 (if the operating pressure and temperature remain the same)? Explain what is meant by the extent of cell disruption is 0.9.
Özcan MM. and his colleagues tried to obtain 3 different fatty acids from moringa pelegrina nucleus with cold pressing and soxhlet extraction by two different methods. The data is as follows; Fatty acids Cold pressing method Moringa pelegrina nucleus Soxhlet extraction method Moringa pelegrina nucleus a-tocopherol(mg/kg) 137.89 ± 2.89 y-tocopherol(mg/kg) 63.68+0.87 8-tocopherol(mg/kg) 151.67+1.89 71.57+1.06 61.49+0.76 63.58+0,97 Accordingly, 3 different fatty acids obtained by 2 different methods used; a) Calculate their efficiency. b) By which method has more efficiency been achieved and explain with the reasons?
3. a. Estimate the molecular weight of sphingosine kinase in kiloDaltons. b. Interpret the results of the experiment with the reducing agent. What does the result of Lane 2 and Lane 3 migrations tell you about the primary, secondary or tertiary structure of the sphingosine kinase?
8L.Part I
Using the information given, determine the Kd for the binding of HABA to BSA. B= y-intercept (E bound) 2457.6x (E unbound) Data for the binding of HABA to BSA y = 4E-06x + 5.2134 R² = 0.928
8) A Leu > Ala mutation at a site buried in the core of the enzyme lysozyme ends up being destabilizing. Explain why in terms of AH", AS" (for both the protein and solvent), and AG".
The following polynucleotide was synthesized and used as a template forpeptide synthesis in a cell-free system from E. coli. …AUAUAUAUAUAUAU… If the same polynucleotide described is used with a mitochondriaderivedcell-free protein-synthesizing system, the product is …Met–Tyr–Met–Tyr–Met–Tyr…What does this say about differences between the mitochondrial and bacterial codes?

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