Using the substrate Ac-Ser-Gln-Asn-Tyr*Pro-Val-Val-NH2 and 10 nM HIV-1 protease, an inhibitor showed competitive inhibition, and a family of lines of 1/v vs. 1/[substrate] at changing-fixed concentrations of this inhibitor were as below: [Inhibitor] (HM Slope (apparent Km/Vmax) (sec) 17,241 21,072 0 10 24,904 20 28,735 30 32,567 40 The Ki value for this inhibitor is
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Using the substrate Ac-Ser-Gln-Asn-Tyr*Pro-Val-Val-NH2 and 10 nM HIV-1 protease, an inhibitor showed competitive inhibition, and a family of lines of 1/v vs. 1/[substrate] at changing-fixed concentrations of this inhibitor were as below:
Slope (apparent Km/Vmax) (sec): 17,241 21,072 24,904 28,735 32,567 [Inhibitor] (M): 0 10 20 30 40
The Ki value for this inhibitor is _________________
![Using the substrate Ac-Ser-Gln-Asn-Tyr*Pro-Val-Val-NH2 and 10 nM HIV-1 protease, an inhibitor showed
competitive inhibition, and a family of lines of 1/v vs. 1/[substrate] at changing-fixed concentrations of
this inhibitor were as below:
[Inhibitor] (HM
Slope (apparent Km/Vmax) (sec)
17,241
21,072
0
10
24,904
20
28,735
30
32,567
40
The Ki value for this inhibitor is](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F224b4099-5fc1-4076-a1db-953078e76a72%2F3e7fb896-9450-4abd-bb22-69af085c4d29%2Fhx8j9ik.png&w=3840&q=75)
Trending now
This is a popular solution!
Step by step
Solved in 7 steps with 6 images
