To determine whether this organism is capable of performing carbohydrate metabolism, ateam of researchers of which you are a part wanted to isolate an enzyme calledglyceraldehyde 3-phosphate dehydrogenase (GAPDH). The enzyme has the followingproperties. Number of amino acids: 335 Molecular Weight: 36,053 DapI: 8.57 Your project leader wanted you to present a basic purification protocol of GAPDH from theorganism based on the given properties above. Give a 1-sentence explanation for each stepof your protocol.
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
To determine whether this organism is capable of performing carbohydrate
team of researchers of which you are a part wanted to isolate an enzyme called
glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The enzyme has the following
properties.
Number of amino acids: 335
Molecular Weight: 36,053 Da
pI: 8.57
Your project leader wanted you to present a basic purification protocol of GAPDH from the
organism based on the given properties above. Give a 1-sentence explanation for each step
of your protocol.
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