Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant DNA technology Microorganism from Type of fragment Enzyme which enzyme is isolated Recognition sequence end produced BamHI Bacillus amyloliquefaciens 5'-GGATCC-3 Cohesive 3'-CCTAGG-3' EcoRI Escherichia coli 5-GAATTC-3 Cohesive 3-CTTAAG-5 EcoRII Escherichia coli 5-CCAGG-3' 3'-GGTCC-5' Cohesive Haelll Haemophilus aegyptius 5'-GGCC-3' 3'-CCGG-5 Blunt HindIll Haemophilus influenzae 5-AAGCTT-3' 3'-TTCGAA-5' Cohesive Notl Nocardia otitidis-caviarum 5-GCGGCCGC-3 Cohesive 3-CGCCGCG-5 Pstl Providencia stuartii 5-CTGCAG-3' 3'-GACGTC-5' Cohesive Smal Serratia marcescens 5-CCCGGG-3' Blunt 3'-GGGCCC-5'
Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant DNA technology Microorganism from Type of fragment Enzyme which enzyme is isolated Recognition sequence end produced BamHI Bacillus amyloliquefaciens 5'-GGATCC-3 Cohesive 3'-CCTAGG-3' EcoRI Escherichia coli 5-GAATTC-3 Cohesive 3-CTTAAG-5 EcoRII Escherichia coli 5-CCAGG-3' 3'-GGTCC-5' Cohesive Haelll Haemophilus aegyptius 5'-GGCC-3' 3'-CCGG-5 Blunt HindIll Haemophilus influenzae 5-AAGCTT-3' 3'-TTCGAA-5' Cohesive Notl Nocardia otitidis-caviarum 5-GCGGCCGC-3 Cohesive 3-CGCCGCG-5 Pstl Providencia stuartii 5-CTGCAG-3' 3'-GACGTC-5' Cohesive Smal Serratia marcescens 5-CCCGGG-3' Blunt 3'-GGGCCC-5'
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter17: Metabolism: An Overview
Section: Chapter Questions
Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...
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Question
The following is the partial sequence of a bacterial gene ORF: 5’ --------------------CGGAATTCCCGGGGATCC------------------------3’ (The remaining sequence of the gene ORF never affects the cloning of this gene. The multiple cloning sites of the plasmid vector can be cleaved by 8 restriction endonucleases that are listed in the table
What is(are) restriction enzyme(s) that cannot be used to insert this bacterial gene ORF into an expression vector below? Explain why
Transcribed Image Text:Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant
DNA technology
Microorganism from
Type of fragment
Enzyme
which enzyme is isolated
Recognition sequence
end produced
BamHI
Bacillus amyloliquefaciens
5'-GGATCC-3
Cohesive
3'-CCTAGG-3'
EcoRI
Escherichia coli
5-GAATTC-3
Cohesive
3-CTTAAG-5
EcoRII
Escherichia coli
5-CCAGG-3'
3'-GGTCC-5'
Cohesive
Haelll
Haemophilus aegyptius
5'-GGCC-3'
3'-CCGG-5
Blunt
HindIll
Haemophilus influenzae
5-AAGCTT-3'
3'-TTCGAA-5'
Cohesive
Notl
Nocardia otitidis-caviarum
5-GCGGCCGC-3
Cohesive
3-CGCCGCG-5
Pstl
Providencia stuartii
5-CTGCAG-3'
3'-GACGTC-5'
Cohesive
Smal
Serratia marcescens
5-CCCGGG-3'
Blunt
3'-GGGCCC-5'
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