Scientists IPed a mutant version of KDM3A with serine 264 altered to aspartic acid (dubbed S/D mutation). They IPed S/D in cells that had not been subjected to heat shock (HS-) or heat shock (HS+). 1) what is the purpose of mutating serine-264 to aspartic acid? 2) what conclusions can scientists make based on results of lane5 and lane 6?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Scientists IPed a mutant version of KDM3A with serine 264 altered to aspartic acid (dubbed S/D mutation). They IPed S/D in cells that had not been subjected to heat shock (HS-) or heat shock (HS+). 1) what is the purpose of mutating serine-264 to aspartic acid? 2) what conclusions can scientists make based on results of lane5 and lane 6?
IP: FLAG-KDM3A
WT
HS
SIA
S/D
Stat1
FLAG
Lane Number:
2
4
5 6
Transcribed Image Text:IP: FLAG-KDM3A WT HS SIA S/D Stat1 FLAG Lane Number: 2 4 5 6
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