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Biochemistry
Zoey Wong is a research officer at the Department of Biosciences of Tunku Abdul Rahman University College. Her supervisor instructs her to prepare chemically competent cells for heat shock transformation from old batches of competent cells that are already available in the freezers. The competent cells will eventually be used for the expression of a prokaryotic enzyme using the pET vector system. (i) (ii) You found several different strains of Escherichia coli in the -80 C freezer. State three (3) E. coli strains you would use for your project which involves the cloning and expression of the protein of interest. In order for cloning and expression to happen optimally, suggest two (2) types of pET vectors suitable for your project.

Zoey Wong is a research officer at the Department of Biosciences of Tunku Abdul Rahman University College. Her supervisor instructs her to prepare chemically competent cells for heat shock transformation from old batches of competent cells that are already available in the freezers. The competent cells will eventually be used for the expression of a prokaryotic enzyme using the pET vector system. (i) (ii) You found several different strains of Escherichia coli in the -80 C freezer. State three (3) E. coli strains you would use for your project which involves the cloning and expression of the protein of interest. In order for cloning and expression to happen optimally, suggest two (2) types of pET vectors suitable for your project.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Zoey Wong is a research officer at the Department of Biosciences of Tunku Abdul Rahman University College. Her supervisor instructs her to prepare chemically competent cells for heat shock transformation from old batches of competent cells that are already available in the freezers. The competent cells will eventually be used for the expression of a prokaryotic enzyme using the pET vector system. (i) (ii) You found several different strains of Escherichia coli in the -80 C freezer. State three (3) E. coli strains you would use for your project which involves the cloning and expression of the protein of interest. In order for cloning and expression to happen optimally, suggest two (2) types of pET vectors suitable for your project.
Transcribed Image Text:Zoey Wong is a research officer at the Department of Biosciences of Tunku Abdul Rahman University College. Her supervisor instructs her to prepare chemically competent cells for heat shock transformation from old batches of competent cells that are already available in the freezers. The competent cells will eventually be used for the expression of a prokaryotic enzyme using the pET vector system. (i) (ii) You found several different strains of Escherichia coli in the -80 C freezer. State three (3) E. coli strains you would use for your project which involves the cloning and expression of the protein of interest. In order for cloning and expression to happen optimally, suggest two (2) types of pET vectors suitable for your project.
Expert Solution
Step 1

The basic principles of the central dogma of molecular biology is similar in both prokaryotic and eukaryotic cells.

This enable cloning an expression of eukaryotic proteins in prokaryotic cells in order to obtain large amounts of the recombinant protein.

The pET expression vector and the BL21 strain of Ecoli forms two essential components of such a prokaryotic expression system.

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