Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as a model system for the study of bacteriophage morphogenesis, DNA replication, andgene regulation. (a)Blunt-end cloning is proven to be more challenging, compared to sticky-end cloning.Illustrate ONE (1) approach that can be conducted in order to perform blunt-end cloning ofa PCR product to a plasmid vector.d) Evaluate the advantages of mRNA as a source material for generating DNA fragments, inrelation to gene cloning.e) E. coli, in most cases is not a suitable host for cloning genes from eukaryotes. Discuss thisstatement.

Q: You have isolated a cDNA clone encoding a protein of interest in a higher eukaryote. This cDNA clone…

A: cDNA  It is known as complementary DNA and made up of reverse transcription of mRNA.

Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: Clearly and neatly diagram an image of the agarose gel indicating the fragments that would be…

A: Electrophoresis It refers to the technique in which the fragments of molecules of DNA, RNA, or…

Q: When a genome is edited using the CRISPR/Cas9 system, a knockout allele can be produced if DNA is…

A: CRISPR: CRISPR  is a newly emerging tool that is used by scientists to alter the genome structure by…

Q: Suggest how the researchers could have altered their targeting construct, to ensure that all of the…

A: To ensure that all of the neomycin resistant transformants would have generated the expected PCR…

Q: Sort the steps to be followed in order to produce human growth hormone (HGH) in the corre Extract…

A: Creating human growth hormone (HGH) using genetic engineering is like a science fiction adventure in…

Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…

A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…

Q: Which restriction enzyme is best suited for cloning in pUC8?

A: The restriction enzymes are specific endonuclease that cut the double stranded DNA from the middle…

Q: Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in…

A: The polymerase chain reaction is the method that is used for making copies of DNA, it is developed…

Q: 3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the…

A: At the permissive temperature, the mutants in dnaB would likely exhibit a normal phenotype since…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: You know from your calculationsthat only a small proportion of the human genome is represented, even…

A: The objective of the question is to outline a strategy for constructing a genomic DNA library that…

Q: the forward primer used in this experiment incorporates part of the cell recognition site, GGCC. How…

A: The TAS2R38 gene in individuals encodes a protein known as taste receptor 2 member 38. TAS2R38 is a…

Q: Why is a thermostable form of DNA polymerase (e.g., Taqpolymerase) used in PCR? Is it necessary to…

A: Polymerase chain response is a typical method used to make numerous duplicates of a specific region…

Q: The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone…

A: A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher…

Q: As you learned in this chapter,restriction enzymes are sophisticated“scissors” that geneticistsand…

A: Making many, exact copies of a specific segment of DNA is known as DNA cloning. The gene or other…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.