You are working as an intern in a research lab. Your supervisor instructs you to prepare electrocompetent cells for transformation from old batches of competent cells. The competent cells would eventually be used for both cloning purposes (to amplify your gene of interest ligated into pUC18). You found several different strains of Escherichia coli in the -80 C freezer: DH5a competent cells One Shot BL21 Star (DE3) competent cells Rosetta™ host strains BL21 competent cells Which ones would be suitable for your work? Justify your selection.

You are working as an intern in a research lab. Your supervisor instructs you to prepare electrocompetent cells for transformation from old batches of competent cells. The competent cells would eventually be used for both cloning purposes (to amplify your gene of interest ligated into pUC18). You found several different strains of Escherichia coli in the -80 C freezer: DH5a competent cells One Shot BL21 Star (DE3) competent cells Rosetta™ host strains BL21 competent cells Which ones would be suitable for your work? Justify your selection.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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The {choose } options are transformation, conjugation, or transduction.
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