Rabbits were in injected with [32 P] photsphate intraperitoneally ans asacrificed after 2, 5, 8, 12, 18, 24, 48, or 168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (cRNA) were prepared from the kidneys and their specific activities (radioactivity/ mg of nucleic acid) were determined 1. what can be concluded from the nRNA and cRNA curves and why 2. why didn thte specific acitivies of nRNA's and cRNA's decrease significantly after 2 days of labeling.
Rabbits were in injected with [32 P] photsphate intraperitoneally ans asacrificed after 2, 5, 8, 12, 18, 24, 48, or 168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (cRNA) were prepared from the kidneys and their specific activities (radioactivity/ mg of nucleic acid) were determined 1. what can be concluded from the nRNA and cRNA curves and why 2. why didn thte specific acitivies of nRNA's and cRNA's decrease significantly after 2 days of labeling.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Rabbits were in injected with [32 P] photsphate intraperitoneally ans asacrificed after 2, 5, 8, 12, 18, 24, 48, or 168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (cRNA) were prepared from the kidneys and their specific activities (radioactivity/ mg of
1. what can be concluded from the nRNA and cRNA curves and why
2. why didn thte specific acitivies of nRNA's and cRNA's decrease significantly after 2 days of labeling.
![EXPERIMENT
Rabbits were injected with [³2P]phosphate intraperitoneally and sacrificed after 2, 5, 8, 12, 18, 24, 48, or
168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (CRNA) were
prepared from the kidneys, and their specific activities (radioactivity/mg of nucleic acid) were
determined.
FIGURE
Relative specific activity
3000
2000
1000
10
20
THE CELL: A MOLECULAR APPROACH 8e, Data Analysis Problem 8.1
2019 Oxford University Press
Kidney
nRNA
CRNA
30
DNA
40
50 Hours
M Kay, and J. N. Davidson. 1965. The
incorporation of radioactive phosphorus into the nucleic acids of different rabbit tissues.
Biochem. J. 60: 177-185.
Source: Smellie, R. M. S., G. H. Humphrey, E. R.
7 Days](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4bc2db8d-b0f4-497e-9433-81c9ca5ab613%2Ff49f2892-b8f8-4e24-b09c-d203f7d44dec%2Fdly270q_processed.png&w=3840&q=75)
Transcribed Image Text:EXPERIMENT
Rabbits were injected with [³2P]phosphate intraperitoneally and sacrificed after 2, 5, 8, 12, 18, 24, 48, or
168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (CRNA) were
prepared from the kidneys, and their specific activities (radioactivity/mg of nucleic acid) were
determined.
FIGURE
Relative specific activity
3000
2000
1000
10
20
THE CELL: A MOLECULAR APPROACH 8e, Data Analysis Problem 8.1
2019 Oxford University Press
Kidney
nRNA
CRNA
30
DNA
40
50 Hours
M Kay, and J. N. Davidson. 1965. The
incorporation of radioactive phosphorus into the nucleic acids of different rabbit tissues.
Biochem. J. 60: 177-185.
Source: Smellie, R. M. S., G. H. Humphrey, E. R.
7 Days
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