5ml of 1 x 10° cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square • Dilution factor: 5 49

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question
5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined
by MTT assay.
a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on
the cell culture plate for the assay. How that cell suspension is prepared if the
following data is given on the cell stock suspension? (6%)
Hemocytometer No. of bright dots
1st large square
52
2nd large square
48
3rd large square
51
4th large square
Dilution factor: 5
49
Transcribed Image Text:5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined by MTT assay. a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square Dilution factor: 5 49
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Hematology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education