Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7 3. Set up controls of the donor and recipient cultures as follows: Spread plate 0.1 ml of the donor culture over the surface of each of the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar + Sm; nutrient agar + Rif) Similarly spread plate 0.1 ml of the recipient culture over the surface of each of the 3 selective media • What is the purpose of this step?

Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7 3. Set up controls of the donor and recipient cultures as follows: Spread plate 0.1 ml of the donor culture over the surface of each of the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar + Sm; nutrient agar + Rif) Similarly spread plate 0.1 ml of the recipient culture over the surface of each of the 3 selective media • What is the purpose of this step?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 5RQ

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