Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7 3. Set up controls of the donor and recipient cultures as follows: Spread plate 0.1 ml of the donor culture over the surface of each of the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar + Sm; nutrient agar + Rif) Similarly spread plate 0.1 ml of the recipient culture over the surface of each of the 3 selective media • What is the purpose of this step?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Diagram illustrating Miles & Misra technique for determining viable counts:
10ul
10-5
106
104
10-7
3. Set up controls of the donor and recipient cultures as follows:
• Spread plate 0.1 ml of the donor culture over the surface of each of
the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar
+ Sm; nutrient agar + Rif)
• Similarly spread plate 0.1 ml of the recipient culture over the
surface of each of the 3 selective media
• What is the purpose of this step?
Transcribed Image Text:Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7 3. Set up controls of the donor and recipient cultures as follows: • Spread plate 0.1 ml of the donor culture over the surface of each of the 3 different selective media (i.e. nutrient agar + Ap; nutrient agar + Sm; nutrient agar + Rif) • Similarly spread plate 0.1 ml of the recipient culture over the surface of each of the 3 selective media • What is the purpose of this step?
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