Q 01 (1 pt.): A variation of centrifugation where the solution in the centrifuge tube varies in density (less dense at the top, more dense at the bottom) is called? А. differential centrifugation B. isopycnic centrifugation C. fractional centrifugation D. variable precipitant centrifugation E. density centrifugation Q 02 (1 pt.): Which of these can either stabilize or disrupt the native fold/structure of a protein? A. solution pH B. temperature C. presence of proteases D. concentration/amount of detergent Е. solution ionic strength Q 03 (5 pt.): Draw: Draw a single/in-solution L- histidine (@ solution pH = 7.5), using a Fischer projection. Make sure to specify all charge states and give the overall charge. (Free gift: pKa1 = 1.8, pKa2 = 9.2, pKa3 = 6.0) ||||| ở |||||
Q 01 (1 pt.): A variation of centrifugation where the solution in the centrifuge tube varies in density (less dense at the top, more dense at the bottom) is called? А. differential centrifugation B. isopycnic centrifugation C. fractional centrifugation D. variable precipitant centrifugation E. density centrifugation Q 02 (1 pt.): Which of these can either stabilize or disrupt the native fold/structure of a protein? A. solution pH B. temperature C. presence of proteases D. concentration/amount of detergent Е. solution ionic strength Q 03 (5 pt.): Draw: Draw a single/in-solution L- histidine (@ solution pH = 7.5), using a Fischer projection. Make sure to specify all charge states and give the overall charge. (Free gift: pKa1 = 1.8, pKa2 = 9.2, pKa3 = 6.0) ||||| ở |||||
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter19: Biomedical Laboratory Instrumentation
Section: Chapter Questions
Problem 2RT
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Transcribed Image Text:Q 01 (1 pt.): A variation of centrifugation where the solution in the centrifuge tube varies in
density (less dense at the top, more dense at the bottom) is called?
differential centrifugation
B. isopycnic centrifugation
C. fractional centrifugation
D.
А.
variable precipitant centrifugation
E. density centrifugation
Q 02 (1 pt.): Which of these can either stabilize or disrupt the native fold/structure of a protein?
solution pH
B. temperature
С.
А.
presence of proteases
D.
concentration/amount of detergent
Е.
solution ionic strength
Q 03 (5 pt.): Draw: Draw a single/in-solution L- histidine (@ solution pH = 7.5), using a Fischer
projection. Make sure to specify all charge states and give the overall charge.
(Free gift: pKa1
3D 1.8, pКa2 %3D9.2, pКа3 3D 6.0)
||| ||
|||||
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