1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml | | ||| 9ml 9ml 9ml 9ml 9ml Chloroform-treated lysate 101 102 103 104 105 0.1 ml 0.1 ml_0.1 ml Empty sterile tubes ||| + 0.5 ml E. coli Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix Molten soft agar overlay O00000 Bottom agar Number of plaque-forming units (pfu) Plate A Dilution Plate B 103 104 105 254 265 132 11 110 23 average number of plaques x dilution factor volume plated pfu ml
1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml | | ||| 9ml 9ml 9ml 9ml 9ml Chloroform-treated lysate 101 102 103 104 105 0.1 ml 0.1 ml_0.1 ml Empty sterile tubes ||| + 0.5 ml E. coli Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix Molten soft agar overlay O00000 Bottom agar Number of plaque-forming units (pfu) Plate A Dilution Plate B 103 104 105 254 265 132 11 110 23 average number of plaques x dilution factor volume plated pfu ml
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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