1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml | | ||| 9ml 9ml 9ml 9ml 9ml Chloroform-treated lysate 101 102 103 104 105 0.1 ml 0.1 ml_0.1 ml Empty sterile tubes ||| + 0.5 ml E. coli Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix Molten soft agar overlay O00000 Bottom agar Number of plaque-forming units (pfu) Plate A Dilution Plate B 103 104 105 254 265 132 11 110 23 average number of plaques x dilution factor volume plated pfu ml

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of
Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate.
1ml
1ml
1ml
1ml
1ml
| | |||
9ml
9ml
9ml
9ml
9ml
Chloroform-treated lysate
101
102
103
104
105
0.1 ml 0.1 ml_0.1 ml
Empty sterile
tubes
|||
+ 0.5 ml E. coli
Incubate inside ref for 15-20min
+ 0.1 ml lysate-E.coli mix
Molten soft agar
overlay
O00000
Bottom agar
Number of plaque-forming units (pfu)
Plate A
Dilution
Plate B
103
104
105
254
265
132
11
110
23
average number of plaques x dilution factor
volume plated
pfu
ml
Transcribed Image Text:1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml | | ||| 9ml 9ml 9ml 9ml 9ml Chloroform-treated lysate 101 102 103 104 105 0.1 ml 0.1 ml_0.1 ml Empty sterile tubes ||| + 0.5 ml E. coli Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix Molten soft agar overlay O00000 Bottom agar Number of plaque-forming units (pfu) Plate A Dilution Plate B 103 104 105 254 265 132 11 110 23 average number of plaques x dilution factor volume plated pfu ml
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