A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity. Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol does not affect them. Which of the control wells will appear blue at the end of the assay? "Live clear" glycerol penicillin + (A1 A2 A3)(A4) bacterial cells (B1 B2)(B3) B4 - А4, B1, B2, ВЗ, В4 А2, А4, B2, В3, В4 A4, B2, B3, B4 A1, АЗ, B1, В2, ВЗ, В4 + | + + + |

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity.
Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in
the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol
does not affect them. Which of the control wells will appear blue at the end of the assay?
"Live clear"
glycerol
penicillin
-
-
+ (A1 A2)(A3)A4)
bacterial cells
B1 (B2 (B3)B4
А4, В1, B2, ВЗ, B4
A2, A4, B2, B3, B4
А4, B2, ВЗ, В4
A1, АЗ, В1, В2, вЗ, В4
+ + |
Transcribed Image Text:A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity. Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol does not affect them. Which of the control wells will appear blue at the end of the assay? "Live clear" glycerol penicillin - - + (A1 A2)(A3)A4) bacterial cells B1 (B2 (B3)B4 А4, В1, B2, ВЗ, B4 A2, A4, B2, B3, B4 А4, B2, ВЗ, В4 A1, АЗ, В1, В2, вЗ, В4 + + |
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