TGF-beta treatment cause cells to grow more or less in the soft-agar assay

What is the difference between in-ovo and ex-ovo types of CAM Assay?

What are the specific E. coli strains used to make competent cells? Explain the characteristics of these strains

what are some advantages to using latex particles instead of blood cells for IM assay?

What is the difference between the total and faecal coliform MPN assay?

Why is it important in this assay to make sure that there is enough bacteria present to produce a lawn? What would happen if you had forgotten to add the E.coil to the soft agar? Why was the water bath set to 50 C? What is the difference between a CFU and a PFU?

What is the purpose of using Triton X in hemolytic assay.

What is micropropogation? Mention its advantages.

Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…

Are the bands indicated in Step 6 (SDS-PAGE) likely visualized by western blot or by a general protein stain (such as Coomassie or silver)?