Question- You have a new microorganism that you have just isolated. You suspect your bacterium is capable of degrading cellulose. You know that these bacteria will have exoglucanases, endoglucanase, and β-glucosidases if they are able to degrade cellulose. 1. What DNA sequencing method would you use? 2. How would you predict the ORFs of the sequence you obtained? 3. How could you predict the functions of those proteins and its metabolic pathways? 4. What enzymes would you look for in the predicted functions of the ORF of the DNA sequence?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question-
You have a new microorganism that you have just isolated. You suspect your bacterium is capable of degrading cellulose. You know that these bacteria will have exoglucanases, endoglucanase, and β-glucosidases if they are able to degrade cellulose.
1. What DNA sequencing method would you use?
2. How would you predict the ORFs of the sequence you obtained?
3. How could you predict the functions of those proteins and its
4. What enzymes would you look for in the predicted functions of the ORF of the DNA sequence?
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