In this gel, the positive and negative ends are indicated at the left of the gel. True or False, the larger DNA pieces are at the top of the gel and the smaller ones are at the bottom. a) True b) False +

In this gel, the positive and negative ends are indicated at the left of the gel. True or False, the larger DNA pieces are at the top of the gel and the smaller ones are at the bottom. a) True b) False +

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A) Which molecule in the gel is the smallest - A, B, C, or D? Explain how you determined this. B) Identify one lane in this gel (1, 2, 3, 4, 5, 6, or 7) that shows evidence of DNase treatment. Explain how you determined this.
the circled answer is wrong.
Below is an image of the results of a gel electrophoresis experiment. Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the DNA size marker (DSM). From this image and by only using Lanes 1- 4 which lane matches each description below? a) This lane contains the largest DNA fragment: b) This lane contains the smallest DNA fragment: c) This lane contains a 2.5 kbp DNA fragment: 42 | || ||| | || 12 kb 9.5 kb 6.5 kb 4 kb 2 kb ***
A student was analyzing the base composition of a DNA sample, but has lost all the data except the content of Cytosine, which is 20%. Based on this information, reconstruct the missing data and list the base composition of the DNA sample.
Is the DNA soluble in the aqueous solution or alcohol?
There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).
How would you estimate the size of the unknown DNA fragement just by looking at the gel?
A DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Length
A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What is it about the chemistry of DNA that causes it to be uniformly negatively charged?
A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What if you had two different DNA fragments that were exactly the same length as measured in base-pairs. Would it be possible to distinguish them using this type of electrophoresis? How would they appear on a gel?
What is the sequence of the unknown DNA sample? (You should give the 5' and 3' ends). Use the gel sequence given below and write also the ingredients used in manual DNA sequencing. Use an empty paper and upload your drawing. || A - T C 0 ||||
Does each sample contain pure DNA? How do you know?