Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqyeness of Reverse Transcriptase PCR (RT-PCR) technique as compared to the common PCR.

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in…

A: Kary Mullis created the groundbreaking technique known as PCR (Polymerase Chain Reaction) in the…

Q: An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the…

A: Every DNA molecule is twisted into a ladder form known as a "double helix." The backbone of the DNA…

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase…

A:

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: FOR PKU:  Draw what you’d expect to see if you were to analyze the gene products by conventional…

A: Analyzing PKU gene products: PCR, RT-PCR, and Western BlotPKU (Phenylketonuria) is an autosomal…

Q: PCR employs in vitro DNA replication. DNA replication in vivo-i.e., in a cell, normally requires…

A: Polymerase chain reaction (PCR) is a method widely used to make many copies of a specific DNA…

Q: Specifically, why is the primer needed in order for DNA ploymerase to start synthesis? A ) primers…

A: Always replication, Transcription starts from 5' to 3'. Addition of nucleotide takes place at 3'-OH.

Q: You are preparing to amplify a DNA sample using PCR and add the following to your set-up: forward…

A: PCR or polymers chain reaction is a process that use to amplify a particular DNA. It needs different…

Q: Describe various ways in which the sequence of DNA is verified or corrected. Why are there so many…

A: Deoxyribonucleic acid or DNA is a molecule comprising of two helical polypeptide strands wound…

Q: A student carries out PCR using the following steps:  step 1: 94C for 1 minute Step 2: 60C for…

A: A complete set of chromosomes in any species is regarded as its genome. Each  chromosomes homes DNA…

Q: In a specific step for PCR, the single-stranded DNA attaches x to primer sequences complementary to…

A: The steps of PCR are - Step 1: Denaturation The DNA double helix separates due to rise in…

Q: The DNA polymerase used in PCR is taken from Thermus aquaticus, a bacterium that thrives in the…

A: The PCR or polymerase chain reaction is the process that produce multiple copy number from a…

Q: 1) polymerize chain reaction is an experimental method for making multiple copies of segments of DNA…

A: Questions :    1) polymerize chain reaction is an experimental method for making multiple copies of…

Q: 3A.Below are a few (of the many) possible errors that could adversely affect PCR or analysis of PCR…

A: A PCR result can be disappointing in many ways. If DNA and primer concentration are reversed, the…

Q: Transformation can be described as:

A: Prokaryotes are unicellular organisms that reproduce in two ways namely- Asexual reproduction and…

Q: 3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the…

A: At the permissive temperature, the mutants in dnaB would likely exhibit a normal phenotype since…

Q: Practical applications of recombinant DNA technology include the: 1. Efficient production of useful…

A: Recombinant DNA technology, also known as genetic engineering, is a technique used to manipulate and…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.