Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqyeness of Reverse Transcriptase PCR (RT-PCR) technique as compared to the common PCR.

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A: PCR is a method used to rapidly make millions of copies of a specific DNA sample and amplify DNA…

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A: Always replication, Transcription starts from 5' to 3'. Addition of nucleotide takes place at 3'-OH.

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Q: molecules continues to double every temperature cycle (referred to as a PCR cycle). (a) Suppose a…

A: Note: We can only answer one question at a time so please resubmit other questions one by one.…

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A: The processivity is defined as the degree of the addition of an average number of nucleotides prior…

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A: Questions :    1) polymerize chain reaction is an experimental method for making multiple copies of…

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A: In the year 1956, Arthur Kornberg isolated and characterized DNA polymerase from E.coli. DNA…

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A: Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity, meaning…

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A: Answer: PCR = Polymerase Chain Reaction is the technique used to amplify the strand of DNA using…

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A: We are answering 1st question only. For the rest of the questions please repost. The polymerase…

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A: Dr.Kary Mullis invented the polymerase chain reaction (PCR) in 1983. The enzyme used in this…

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A: PCR - Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing…

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A: PCR is a technique that results in exponential amplification of a selected region of a DNA molecule…

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A: The polymerase chain reaction is abbreviated as PCR. It is a molecular biology technique for…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.