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30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon

30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon
Transcribed Image Text:30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon
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