Homework Help is Here – Start Your Trial Now!
Science
Biology
30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon

30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Question 39

30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon
Transcribed Image Text:30. Explain subtractive cloning, and how it was used to discover the location of the Duchenne Muscular Dystrophy gene (What was sonicated? What was digested? What was cloned and sequenced?).- why wouldn't this work with most other genes (like Cystic Fibrosis, which results from the deletion of a singie codon)? • 31. If you digested the human genome with EcoRI and ran it on an agarose gel, what would you see? Wnyr What would you need to do to "find" the gene of interest? What is this technique calied? • 32. How was RFLP analysis used to locate a phenotype/gene to a specific locus in the human genome, such as in Huntington's Disease, Marfan's, CF, etc.? 33. Why can A phage be used to take up sections of the human genome? 34. What are the two limits of using genomic libraries to find specific genes? • 35. How do genomic and CDNA libraries differ? Which can be screened with antibodies and why? • 36. How are oligonucleotides, once important as probes for screening libraries, used today? • 37. What is the difference between cioning and PCR? 38. Why did scientists push for the human genome to be sequenced? • 39. What modification of the nucleotides allowed for Sanger Sequencing? What effect do these nucleotides have on replicating DNA? 40. Compare chromosomal walking and shotgun sequencing. Which was more successful, and why? Recommended Questions: These questions are not mandatory to complete but are recommended to aid in your study and preparation for the exam. Chapter 6. 41. Define the following domains: SH2, SH3, Bromo, Chromo, PTB, SNARE, EF-Hand 42. Describe the role/impact of each, and any important amino acids involved in the process: a. Allostery b. Conformational changes c. "And" and "or" gates d. Acetylation Glucon
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Analytical Methods
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS