Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 9197) pRS-marker
Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 9197) pRS-marker
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:bGH poly(A) signal
(6977 6993) M13 Reverse
PCDNA3.1-
9202 bp
(6939) BstZ171
(6898 . 6917) EBV-rev
(6887) Bsml
(6844 6863) SV40PA-R
(6649) BstBI
(6503 6522) Neo-F
(6483) Rsril
SV40 ori
(6364) BSSHII
SV40 promoter
PpuMI (3289)
(5893 5912) Neo-R
fl ori
(5780) Smal
(5778) TspMH - Xmal
(5757) Avrll
(5756) Stul
14000
15000
(5685. 5704) SV40pro-F
BbvCI (3798)
(5438 5458) PBABE 3
(5298 5319) Flori-F
(5088 . 5107) Flori-R
(4725 4742) BGH-rev
(4709) Pmel
(4704) Apal
(4700) PSPOMI
PaeR71 PspXI Xhol (4688)
Xbal (4694)
13000
M13 rev
d bej | signal
R/KanR
6000
Transcribed Image Text:QUESTION 2
The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral
protein (current infection).
You plan to express the mutant spike protein variant using the plasmid pictured below as your template.
Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy
as a gene block! But you need to add a couple things to it first so it'll get into the plasmid.
To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867).
Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first
blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site
name you'd add at the end of the gene (N- terminus end).
(9178 .. 9197) pRS-marker
Nrul (140)
(9132) SgrDI
(9015) Sspl
Mlul (160)
Spel (181)
Ndel (416)
SnaBI (522)
(8761 .. 8780) Amp-R
(8581) Pvul
CMV-F (701 .. 721)
Eco53kl (748)
Sacl (750)
9000
T7 (795.. 814)
Nhel (827)
Bmtl (831)
BsmBI (873)
CMV en...
1000
CMV promoter
T7 promoter
747R1RD322oriF
10008
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