QUESTION 9In our walk-through of the HIV ELISA in zoom class, we were doing a test used to diagnose HIV. How would the procedure be different if we were testing for antibodyto West Nile virus (WNV)?WNV antigen would be adsorbed to the wellsa different enzyme would be attached to the secondary antibodyⒸanti-WNV antibodies would be adsorbed to the wellsenzyme-linked anti-WNV antibody would be used as the secondary antibodyO a different substrate would be used

ELISA stands for Enzyme Linked Immunosorbent Assay. It is a common laboratory method in molecular…

Answered: QUESTION 9 In our walk-through of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 23 BONUS: Match the viral adhesin to it cells receptor influenza A virus Hemagglutinin [Choose] [Choose J COVID 19 pandemic coronavirus S protein sialic acid receptors ACE-2 receptors in lungs
QUESTION 1 SDS-PAGE reagents that play a role in formation of the gel only are(Select all that applies) Beta-Mercaptoethanol Bromophenol blue APS Heat Sodium Dodecyl Sulfate Bis-acrylamide Acrylamide TEMED 3.33 points QUESTION 2 In Lab 3 we aimed to separate lysozyme from the Hen egg white proteins. Which of the following is the correct observation/result from that lab? Load contains all the neutral and negatively charged proteins. HEW contains negative and neutral proteins only. Carb 1 contains negatively charged proteins. Load contains positive, negative and neutral proteins. 3.34 points QUESTION 3 In order to separate negatively charged proteins from a mixture of neutral, positively charged and negatively proteins, you should pass the mixture of these beads through Gel filtration column, using anion…
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 11 Which of the following is not an antiviral drug? O Protease inhibitors O Fusion inhibitors O Cell wall synthesis inhibitors O Entry inhibitors QUESTION 12 Which of the following does not contribute to antibiotic resistance? O Consuming antibiotics in food O Finishing a full antibiotic prescription O Not finishing your antibiotics because you feel better O Using antibiotics for conditions other than a bacterial infection Click Save and Submit to save and submit. Click Save All Ansuwers to save all answers. P Type here to search 立

QUESTION 8 Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. O A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. O B. Whole genome sequencing files will have more reads in them because genomes are bigger than 165 amplicons. O C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. O D. The 165 molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. O E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. O F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.
Question 1 Listen If you are given a sample with 1,000,000 pfu/mL of bacteriophage (Tube A) and plate 1mL of the sample on a TSA plate, what will you observe after 24 hours incubation? (How many plaques?) 100,000 because it is a 1:10 dilution. Zero because bacteriophage need a host to grow. More than 300 because it will be TNTC. 1,000,000 plaques because there are 1,000,000 phage in 1mL.
Question 2
QUESTION 8 Under which condition(s) can a disease become an epidemic? Choose all that apply. Ro = 0.8 Ro = 1 Ro = 1.1 %3D Ro = 3
QUESTION 18 You are studying an emerging pathogen. You discover that patients have been infected up to twenty years previously with the pathogen though they did not develop signs and Upon further analysis you note that their major organ systems have been slowly damaged by the pathogen over time. You will document this pathogen as causing which type of infection? latent zoonotic acute O chranic QUESTION 19 Which of the following is a type of membrane disrupting toxin? O a. superantigen O b. botulinum toxin Oc coagulase O d. phosopholipase QUESTION 20 Which of the following is an example of an exoenzyme that can allow microbes to be more invasive? O a. hyaluronidase O b.coagulase Oc ipid A O d. superantigen
QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal
Question 1 A 60-year-old woman was recently given a diagnosis of advanced non- small cell lung cancer. She is going to begin treatment with cisplatin 100 mg/m 2 plus vinorelbine 30 mg/m2. Discuss your pharmacological plan
Question 20 Drugs that target viral gene expression must accomplish functional in human hosts. Block viral entry Reverse transcriptase Block viral genetic takeover All of the above in order to be

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