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- QUESTION 10 The drug shown in the figure below is an anticancer agent that disrupts the overall chromatin structure by binding AT-region regions of DNA and preventing the interactions shown in the figure below. The region A site shown below are called [x]? H OH HN HN HO NH Region A? AT-specific drug cooooo Nuclear matrix Transcription/replication machineryhelp meQuestion 7. What is the sequence of the primary transcript produced from this gene? -35 sequence Pribnow box 5' GATTCCGTATTACAGCATAGGCTATATTCACGTGGTACGCTA 3' 3' CTAAGGCATAATGTCGTATCGATATAAGTGCACCATGCGAT 5' Start site Short Answer 140
- Please answer part 2 and 3, not graded questions,thxQUESTION 1 SDS-PAGE reagents that play a role in formation of the gel only are(Select all that applies) Beta-Mercaptoethanol Bromophenol blue APS Heat Sodium Dodecyl Sulfate Bis-acrylamide Acrylamide TEMED 3.33 points QUESTION 2 In Lab 3 we aimed to separate lysozyme from the Hen egg white proteins. Which of the following is the correct observation/result from that lab? Load contains all the neutral and negatively charged proteins. HEW contains negative and neutral proteins only. Carb 1 contains negatively charged proteins. Load contains positive, negative and neutral proteins. 3.34 points QUESTION 3 In order to separate negatively charged proteins from a mixture of neutral, positively charged and negatively proteins, you should pass the mixture of these beads through Gel filtration column, using anion…QUESTION 13 A scientist would like to overexpress a lysosomal acid hydrolase in a cultured cell line as a way to investigate a potential therapy for a lysosomal storage disease. How might this scientist introduce a plasmid encoding this lysosomal enzyme into cells? O Scanning electron microscopy O Western blot analysis O Transfection with a lipid reagent (AKA liposomes) O Immunohistochemistry O Generate an organoid QUESTION 14 Heterotrimeric G-proteins that associate with the G-protein coupled receptors (GPCRs) consist of which of the following? OA. beta subunits C B. O C. alpha subunits alpha, beta, and gamma subuni OD. beta and gamma subunits O E. gamma subunits QUESTION 15 The activation of a common effector molecule in the cytoplasm by signals from a variety of unrelated receptors is called OA. Divergence OB. Convergence OC. Crosstalk OD. Transvergence QUESTION 16
- QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…QUESTION 51 CRISPR is a revolutionary gene editing technology that could potentially be used for human germline editing. Human germline editing is the process by which the genome of an individual is altered in such a way that the change is heritable (can be passed on to offspring). This is achieved through genetic alterations within the germ cells. While many agree that somatic cell editing may be ethical for use in combating cancer and other chronic diseases, many countries now prohibit by law the use of germline editing. 1. Discuss the difference between somatic cells and germ cells. 2. Offer your opinion of why germline editing should be, and also why it should not be, permitted by law. (6 sentences minimum). Make sure to offer points on both sides regardless of your personal opinion. T TTT Paragraph v Arial 3 (12pt) 回i 会 X D O Q T T O f O Mashups 曲 E E E HO I HTML CSSWhich feature is associated with antisocial personality disorder? elevated resting heart rate lower resting heart rate enlarged first and third ventricles diminished first and third ventricles
- Question 25 In all types of splicing a 2' OH form an A in the branch point attacks the 5' splice site the 3'OH from the 1st exon attacks the 3' splice site the 3' OH from the 1st exon attacks the 5' splice site O none of the above Moving to another question will save this response. MacBook F 00OQUESTION 10 Remdesivir is converted in vivo to CMP analog and then phosphorylated to CTP analog. a successful treatment for Ebola. There are more than 1 correct answers in the other choices. a potential chain terminator for the replication of RNA viral genomeQuestion 1. Describe and explain the epidemiological evidence supporting the view that cancer develops through a multi-step process involving increasingly severe stages.. Question 2. Describe and explain the genetic evidence supporting the view that cancer develops through a multi-step process involving increasingly severe stages.