Question 12. After transformation by electroporation, you can select transformed bacteria that carry a plasmid with an insert because: a. The transformed bacterial colony will be able to grow in the presence of ampicillin. b. The transformed bacterial colony will die in the presence of ampicillin. c. The transformed bacterial colony will be able to grow in the presence of ampicillin and will NOT have ß-galactosidase activity (white colony). d. The transformed bacterial colony will be able to grow in the presence of ampicillin and WILL have ß-galactosidase activity (blue colony). e. The transformed bacterial colony will die in the presence of ampicillin and will not have ß-galactosidase activity (a white colony on LB plates, minus ampicillin).
Question 12. After transformation by electroporation, you can select transformed bacteria that carry a plasmid with an insert because: a. The transformed bacterial colony will be able to grow in the presence of ampicillin. b. The transformed bacterial colony will die in the presence of ampicillin. c. The transformed bacterial colony will be able to grow in the presence of ampicillin and will NOT have ß-galactosidase activity (white colony). d. The transformed bacterial colony will be able to grow in the presence of ampicillin and WILL have ß-galactosidase activity (blue colony). e. The transformed bacterial colony will die in the presence of ampicillin and will not have ß-galactosidase activity (a white colony on LB plates, minus ampicillin).
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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Transcribed Image Text:Question 12. After transformation by electroporation, you can select transformed bacteria
that carry a plasmid with an insert because:
a. The transformed bacterial colony will be able to grow in the presence of
ampicillin.
b. The transformed bacterial colony will die in the presence of ampicillin.
c.
The transformed bacterial colony will be able to grow in the presence of
ampicillin and will NOT have ß-galactosidase activity (white colony).
d. The transformed bacterial colony will be able to grow in the presence of
ampicillin and WILL have ß-galactosidase activity (blue colony).
e. The transformed bacterial colony will die in the presence of ampicillin and
will not have ß-galactosidase activity (a white colony on LB plates, minus
ampicillin).
4
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