Question 13. After electroporating 20 ng of your plasmid into bacterial cells and recovering the cells for 45 minutes at 37°C, you made a 1:100 dilution of the cell culture. you plated 100 μl on a medium supplemented with IPTG, X-gal, and ampicillin. The next morning you counted 150 white and 50 blue colonies on your plate. How many of the transformants contain your DNA insert? a. 15 colonies. b. 200 colonies. 50 colonies. C.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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