Question 3ListenImagine that you took a stock of 1,000,000 pfu/mL of bacteriophage and made a series of dilutions by taking 1mLfrom the stock and adding it to 9mL of fresh broth. You then mixed that tube and repeated 2 more times, each timetaking 1mL and adding it to 9mL of fresh broth, for a total of 3 dilutions. THEN you added a few drops of E. coli andplated 1mL from your final tube on a TSA plate. What would you observe after 24 hours incubation? It might help todraw a picture. (How many plaques?)1101001,00010,000100,0001,000,000

In this case, we are examining a bacteriophage stock dilution setup, followed by E. coli infection…

Answered: Question 3 Listen Imagine that you took…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question 1 Listen If you are given a sample with 1,000,000 pfu/mL of bacteriophage (Tube A) and plate 1mL of the sample on a TSA plate, what will you observe after 24 hours incubation? (How many plaques?) 100,000 because it is a 1:10 dilution. Zero because bacteriophage need a host to grow. More than 300 because it will be TNTC. 1,000,000 plaques because there are 1,000,000 phage in 1mL.
Question 4. You are analyzing cells that you have grown on coverslips and fixed. What method would you use to determine where in the cell a protein is located? A. Western blot B. SDS-PAGE C. Microarray D. Immnocytochemstry E. Single cell RNA sequencing
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