Polyprotic acids such as H3PO4, can act asacid-base buffersonly in combination with polyprotic basesB if their concentration is kept lowat pH values around neutralityat pH values around any of their pka's

D. at pH values around any of their pKa's. Polyprotic acids are acids that can donate more than one…

Answered: Polyprotic acids such as H3PO4, can act…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Benzalkonium chloride (E=0.16) 1.5g qs 100 ml Distilled water 0.9% Nacl solution ad Make isotonic solution Questions: 1. How many ml of distilled water must be added to make the Benzalkonium chloride preparation isotonic? 2. How many ml of 0.9% NaCl must be added to make a 100ml solution?
Give the complete solution.
what is the pH of a solution with a H+ concentration of 1.44 x 10-3 M
Zwitter ions contain an equal number of ionizable groups of opposite charge and that bear no net charge True False
Which of the following is TRUE if pH is higher than the pka of the buffer? O [weak acid] approximately 0 O [conjugate base] = [weak acid] [conjugate base] > [weak acid] O [conjugate base] < [weak acid]
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
The absorbance values for different BSA standards. How can the protein concentration be calculated? Which absorbance value should be used?
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pH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)
An appropriate biochemical buffer should have charges in both its conjugate acid and conjugate base form. This avoids the presence of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? O Yes No What about buffering at pH 4? D Yes No
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Polyprotic acids such as H3PO4, can act as acid-base buffers ℗ only in combination with polyprotic bases Ⓡ if their concentration is kept low at pH values around neutrality