The 3 proteins have the following properties. Which is the correct order of steps to extract them?+ ethyl acetate, + (NH4)2SO4 at 20% saturation, centrifugation, + detergent (Triton X), buffer the lysate (pH=10), centrifugation, collect precipitate+ ethyl acetate, + detergent (Triton X), + (NH4)2SO4 at 20% saturation, centrifugation, buffer the lysate (pH=10), centrifugation, collect precipitate+ 1 M NaCl, + detergent (sodium dodecyI suIfate), centrifugation, +(NH4)2SO4 at 60% saturation, buffer at pH 7, centrifugation, collect precipitate+ ethyl acetate, adjust pH to 7.3, centrifugation, collect supernate, +detergent (Triton X) to precipitate, +(NH4)2SO4 at 30% saturation, centrifugation, collect precipitate % (NH4)2SO4 toprecipitateProteinplLocation7.0Mitochondrial matrix 307.3Cytosol209.8Mitochondrial matrix 60

Protein extraction is a molecular biological method to isolate a particular one or two proteins…

Answered: The 3 proteins have the following…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

The 3 proteins have the following properties. Which is the correct order of steps to extract them?

+ ethyl acetate, + (NH₄)₂SO₄at 20% saturation, centrifugation, + detergent (Triton X), buffer the lysate (pH=10), centrifugation, collect precipitate
+ ethyl acetate, + detergent (Triton X), + (NH₄)₂SO₄at 20% saturation, centrifugation, buffer the lysate (pH=10), centrifugation, collect precipitate
+ 1 M NaCl, + detergent (sodium dodecyI suIfate), centrifugation, +(NH₄)₂SO₄at 60% saturation, buffer at pH 7, centrifugation, collect precipitate
+ ethyl acetate, adjust pH to 7.3, centrifugation, collect supernate, +detergent (Triton X) to precipitate, +(NH₄)₂SO₄at 30% saturation, centrifugation, collect precipitate