My boss has given me the purified extracellular portion of the Notch receptor. As Delta/Notch signaling occurs between cells, I know that both the Notch receptor and and Delta are most likely "comfortable" in the environment of the blood, not the cytosol, nucleus, mitochondria, etc... I want to purify Delta, the signal. What would be true of the most specific purification technique I could choose, i.e. the technique that will result in the fewest proteins in the fraction containing Delta? Do not worry about whether the protein works after fractionation yet. Pick ALL that apply.    I would use ion exchange chromatography.            I would use size exclusion chromatography            I would use affinity chromatography            I would use a water based mobile phase of 7.4            I would use an acetonitrile mobile phase with a pH of 7.4        I would use a water based mobile phase of pH 7.2            I would use an acetonitrile phase of 7.2         I would salt it out.            I would obtain my soluble protein fraction by dialysis.            I would obtain my soluble protein fraction by eluting from the column in stages slowly changing the pH.

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Cell Signaling

It allows cells to perform various cellular activities, coordinate, and interact with other cells. All cells of an organism receive signals from their environments and respond accordingly. Even the unicellular eukaryotes receive signals secreted by other cells and respond. In the case of yeast, mating takes place by signaling between the yeast cells. Communication by cell signaling in a multicellular organism has become a vast and important part of various fields of biology.