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rk band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB !! !== phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply.

rk band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB !! !== phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1 of 16 Data from an experiment is shown in the figure below. In the experiment, murine cells were treated with a specific a ligand that activates receptor R. In some cases, the cells were exposed to one of two drugs (X or Y) as well as the ligand or were left untreated (UT). After 30 minutes of treatment, the cells were lysed with a detergent-based buffer to release the soluble membrane, cyosolic and nuclear proteins. Samples from each cell extract were run (in duplicate) by SDS-PAGE (SDS-polyacrylamide gel electrophoresis) in order to separate the proteins by molecular mass (size). The separated proteins were then transferred to a nitrocellulose membrane which was then probed with different antibodies in a western blotting procedure to detect specific phosphorylated proteins or total proteins. If present in the cell extract, these proteins appear as a dark band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply. The MAPK pathway The protein Kinase A pathway AIl of these
Transcribed Image Text:1 of 16 Data from an experiment is shown in the figure below. In the experiment, murine cells were treated with a specific a ligand that activates receptor R. In some cases, the cells were exposed to one of two drugs (X or Y) as well as the ligand or were left untreated (UT). After 30 minutes of treatment, the cells were lysed with a detergent-based buffer to release the soluble membrane, cyosolic and nuclear proteins. Samples from each cell extract were run (in duplicate) by SDS-PAGE (SDS-polyacrylamide gel electrophoresis) in order to separate the proteins by molecular mass (size). The separated proteins were then transferred to a nitrocellulose membrane which was then probed with different antibodies in a western blotting procedure to detect specific phosphorylated proteins or total proteins. If present in the cell extract, these proteins appear as a dark band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply. The MAPK pathway The protein Kinase A pathway AIl of these
Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply. The MAPK pathway The protein Kinase A pathway All of these The Phospholipase C pathway None of these Submit
Transcribed Image Text:Drug X Drug Y UT Ligand Ligand Ligand Western blotting antibodies phospho-CREB phospho-ERK1/phospho-ERK2 phospho-PKC Total GAPDH You should assume (i) that any phosphorylation event occurs as the results of the action of an upstream enzyme and (ii) that protein phosphorylation causes the activation of that protein. Which intracellular signalling pathways does the ligand activate in these cells? Select all answers that apply. The MAPK pathway The protein Kinase A pathway All of these The Phospholipase C pathway None of these Submit
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