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IDENTIFY THE METHOD. FIGURE 1. IL-1ß increases protein expression of PDGFRB and CD13 in cultured pericytes. The researchers used what method to generate the results in A? A PDGFRB a-tubulin D CD13 B-actin P-AKT total AKT P-ERK total ERK GAPDH IL-18 05 10 50 IL-18 0 5 10 50 B E PDGFRB/a-tubulin p-/total AKT 1.5 1.0- 0.5- 1.51 1.0- 0.5- 0 5 10 50 IL-18 (ng/ml) 0 5 10 IL-18 (ng/ml) 50 с CD13/B-actin F p-total ERK 2.01 1.5- 1.0- 0.5- 0 1.5- 1.0- 0.5- 0 H 10 50 5 IL-13 (ng/ml) 0 5 10 IL-13 (ng/ml) 50

IDENTIFY THE METHOD. FIGURE 1. IL-1ß increases protein expression of PDGFRB and CD13 in cultured pericytes. The researchers used what method to generate the results in A? A PDGFRB a-tubulin D CD13 B-actin P-AKT total AKT P-ERK total ERK GAPDH IL-18 05 10 50 IL-18 0 5 10 50 B E PDGFRB/a-tubulin p-/total AKT 1.5 1.0- 0.5- 1.51 1.0- 0.5- 0 5 10 50 IL-18 (ng/ml) 0 5 10 IL-18 (ng/ml) 50 с CD13/B-actin F p-total ERK 2.01 1.5- 1.0- 0.5- 0 1.5- 1.0- 0.5- 0 H 10 50 5 IL-13 (ng/ml) 0 5 10 IL-13 (ng/ml) 50

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**IDENTIFY THE METHOD.** **FIGURE 1. IL-1β increases protein expression of PDGFRβ and CD13 in cultured pericytes. The researchers used what method to generate the results in A?** **Description:** **Panel A:** Shows a Western blot analysis indicating the protein levels of PDGFRβ and CD13 in the presence of varying concentrations of IL-1β (0, 5, 10, 50 ng/ml). The loading controls are α-tubulin and β-actin. **Panel B:** A bar graph representing the ratio of PDGFRβ to α-tubulin at different IL-1β concentrations. The graph indicates a significant increase in PDGFRβ expression with higher IL-1β concentrations. Statistical significance is denoted by asterisks (* p < 0.05, ** p < 0.01). **Panel C:** A bar graph depicting the ratio of CD13 to β-actin with increasing IL-1β concentrations. Similar to Panel B, there is a notable increase in CD13 expression as IL-1β concentration rises. **Panel D:** Presents another set of Western blots for phosphorylated AKT (p-AKT), total AKT, phosphorylated ERK (p-ERK), total ERK, with GAPDH as the loading control. **Panel E:** Shows a bar graph of the p-AKT to total AKT ratio. The results illustrate a significant elevation at higher IL-1β concentrations. **Panel F:** A bar graph of the p-ERK to total ERK ratio, indicating a similar trend of increased phosphorylation with increasing IL-1β. These figures together suggest that IL-1β promotes the expression and activation of specific proteins in cultured pericytes, as assessed by Western blot analysis. The statistical significance is marked, indicating reliable and reproducible results.
Transcribed Image Text:**IDENTIFY THE METHOD.** **FIGURE 1. IL-1β increases protein expression of PDGFRβ and CD13 in cultured pericytes. The researchers used what method to generate the results in A?** **Description:** **Panel A:** Shows a Western blot analysis indicating the protein levels of PDGFRβ and CD13 in the presence of varying concentrations of IL-1β (0, 5, 10, 50 ng/ml). The loading controls are α-tubulin and β-actin. **Panel B:** A bar graph representing the ratio of PDGFRβ to α-tubulin at different IL-1β concentrations. The graph indicates a significant increase in PDGFRβ expression with higher IL-1β concentrations. Statistical significance is denoted by asterisks (* p < 0.05, ** p < 0.01). **Panel C:** A bar graph depicting the ratio of CD13 to β-actin with increasing IL-1β concentrations. Similar to Panel B, there is a notable increase in CD13 expression as IL-1β concentration rises. **Panel D:** Presents another set of Western blots for phosphorylated AKT (p-AKT), total AKT, phosphorylated ERK (p-ERK), total ERK, with GAPDH as the loading control. **Panel E:** Shows a bar graph of the p-AKT to total AKT ratio. The results illustrate a significant elevation at higher IL-1β concentrations. **Panel F:** A bar graph of the p-ERK to total ERK ratio, indicating a similar trend of increased phosphorylation with increasing IL-1β. These figures together suggest that IL-1β promotes the expression and activation of specific proteins in cultured pericytes, as assessed by Western blot analysis. The statistical significance is marked, indicating reliable and reproducible results.
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