= Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Complete the following problems. Sign in Find text or tools H Restriction enzymes (RES), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, PMBBS, through restriction digests. You subjected the PMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and XhoI), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol 1+1 +1+ DNA ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp *The same total amount of DNA was loaded in each lane. 1. What is the total size of the pMBBS plasmid in bp? Answer: bp 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: VC 100bp Plus DNA ladder from Vivantis. Technologies Q Search IA W ☑ 0 Al Assistant Dr. 1 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q = Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Sign in Find text or tools H 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes: 4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in Figure 1. Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.) Answer: (provide a drawing) NFL Q Search IA W ☑ 0 Al Assistant C 2 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q
= Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Complete the following problems. Sign in Find text or tools H Restriction enzymes (RES), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, PMBBS, through restriction digests. You subjected the PMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and XhoI), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol 1+1 +1+ DNA ladder 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp *The same total amount of DNA was loaded in each lane. 1. What is the total size of the pMBBS plasmid in bp? Answer: bp 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: VC 100bp Plus DNA ladder from Vivantis. Technologies Q Search IA W ☑ 0 Al Assistant Dr. 1 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q = Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Sign in Find text or tools H 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes: 4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in Figure 1. Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.) Answer: (provide a drawing) NFL Q Search IA W ☑ 0 Al Assistant C 2 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:= Menu
#1 Mol Bio Restriction An... X
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Complete the following problems.
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Find text or tools H
Restriction enzymes (RES), which cut D NA at specific sequences, are classic tools in molecular
biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences
by analyzing the fragments generated upon restriction digest, as in the example shown in Figure
1. Your task is to study the circular plasmid, PMBBS, through restriction digests. You subjected
the PMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI,
and XhoI), and analyzed the results on an agarose gel, shown below. Using the data you can glean
from this gel, answer the questions that follow.
EcoRI
BamHI
Xhol
1+1
+1+
DNA
ladder
3000 bp
2500 bp
2000 bp
1500 bp
1200 bp
1000 bp
900 bp
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp
*The same total amount of DNA was loaded in each lane.
1. What is the total size of the pMBBS plasmid in bp?
Answer:
bp
2. How many cut sites on the PMBBS plasmid does each RE have?
EcoRI:
BamHI:
Xhol:
VC 100bp Plus DNA ladder
from Vivantis. Technologies
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W
☑
0
Al Assistant
Dr.
1
2
C
1:1
༢
☑
8:58 PM
10/22/2024
Q
Transcribed Image Text:= Menu
#1 Mol Bio Restriction An... X
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3. If the PMBBS plasmid were digested with all three REs together, how many fragments would
be observed, and what would be the sizes (in bp) of these fragments?
Number of fragments:
Sizes:
4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in
Figure 1. Make sure you show the following information: (1) relative locations of the RE sites; (2)
size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.)
Answer: (provide a drawing)
NFL
Q Search
IA
W
☑
0
Al Assistant
C
2
2
C
1:1
༢
☑
8:58 PM
10/22/2024
Q
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