EcoRI BamHI Xhol III +11 1+1 11+ 1++ +1+ ++ 1 DNA size ladder *The same total amount of DNA was loaded in each lane. 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp VC 100bp Plus DNA ladder from Vivantis Technologies

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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 pMBBS plasmid was subjected to complete digestion by different combinations of three restrictive enzymes (EcoRI, BamHI, and XhoI), and the results analyzed  on an agarose gel, Figure 1. Using the data, respond to the question below

  1. Draw the actual map of the pMBBS plasmid, following the style of the sample map shown in Figure Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.)

     

EcoRI
BamHI
Xhol
III
+11
1+1
11+
1++
+1+
++ 1
DNA
size
ladder
*The same total amount of DNA was loaded in each lane.
3000 bp
2500 bp
2000 bp
1500 bp
1200 bp
1000 bp
900 bp
800 bp
700 bp
600 bp
500 bp
400 bp
300 bp
200 bp
100 bp
VC 100bp Plus DNA ladder
from Vivantis Technologies
Transcribed Image Text:EcoRI BamHI Xhol III +11 1+1 11+ 1++ +1+ ++ 1 DNA size ladder *The same total amount of DNA was loaded in each lane. 3000 bp 2500 bp 2000 bp 1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp VC 100bp Plus DNA ladder from Vivantis Technologies
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