The other question is, how many CT value differences is acceptable for duplication?

Q: Which would you choose if you needed to make a partial diploid for complementation analysis? rolling…

A: The correct answer is option 1. Rolling circle plasmid.

Q: The following three questions are related to this patient scenario: A 65-year-old male HIV patient…

A: The intravenous antibiotics are used to treat severe kinds of bacterial infections. A most common…

Q: There are two bacteria, and I have to find out the organism. I did gram staining, and I found out…

A: Gram staining is a laboratory technique used to differentiate and classify bacteria into two major…

Q: To determine whether proteins interact with each other which two techniques might you use 1. Yeast…

A: Protein-protein interactions (PPIs) are highly-specific physical contacts that occur between two or…

Q: Each of the three different Hfr strains in the table below (A, B and C) arose independently and…

A: A genome is the complete set of genetic information in an organism. It provides all of the…

Q: a) Based on your calculations of the DNA concentrations, how much DNA was loaded into each well? Do…

A: DNA yeild is measured on basis of absorbance.  At A260 absorbance DNA absorbs light most strongly,…

Q: For each tube in a serial dilution, 1 ml of one solution is diluted into 9 ml H₂O. ● Determine the…

A: In serial dilution, the density of the cells is decreased at each step so that the concentration of…

Q: Why does it make sense to look for ARGs and not antibiotic-resistant organisms themselves?

A: Antibiotic resistance genes (ARGs) are segments of DNA that encode for proteins or enzymes that…

Q: You will be given the plaque assay plates to analyse from three different bacteriophages stocks You…

A: It's crucial to select the best phage for the job while carrying out mutagenesis research utilising…

Q: On the four compounds testes for antibacterial activity using agar well diffusion assay, a. Which…

A: Antibacterial is an activity performed by any chemical or other compound through which it will the…

Q: Using phages that came from E. coli with the genotype al+ be+, you infect a strain ofE. coli that is…

A: Auxotrophs are the term used for the organism or bacteria that cannot express a gene due to mutation…

Q: AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST

A: Bacitracin Test --  Bacitracin is a bactericidal drug useful in the treatment of superficial skin…

Q: You are counting plaques on your plaque assay plates made from serial dilutions of your high titer…

A: A serial dilution is any dilution in which the concentration decreases by the same dilutions in each…

Q: The PCB concentration in Lake Michigan is declining. The concentration can be modeled using a first…

A: The first order reaction is a reaction that proceeds at the rate that depends linearly on only one…

Q: In a plaque assay, a phage has undergone _________ cycle when plaques are observed on the agar lawn…

A: Viruses are obligate intracellular parasites that requires a living host cell to grow and multiply.…

Q: whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR?…

A: Bacterial vaginosis is caused by multiple types of  germs including Mycoplasma and bacteria dwelling…

Q: Is the isolation plate pigmented? (is their a color for it)? what is colony morphology(single cells…

A: Colony morphology is a method that is used to describe the characteristics of an individual colony…

Q: Looking at the model data i upload and given the question how could i analyse tboth experimental…

A: Agarose gel electrophoresis is the separation of DNA on the basis of size, supercoiled DNA will move…

Q: 12. When a person is determining the concentration of a sample, they will frequently repeat the same…

A: Introduction The repeated analysis of a single sample is a measure of the greatest precession…

Q: Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a…

A: Conjugation is the process of genetic material transfer between two bacterial cells through direct…

Q: If the concentration of phage in the original sample was markedly increased from the concentration…

A: 1. Dilution Series:  - Start with a high dilution, such as 10^-5 or 10^-6, of the original phage…

Q: Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some…

A: The melting curves are generated when the DNA is heated along with the fluorescent DNA-binding dye.…

Q: Please answer the following using the experiment data below. Provide the formulas or methods used…

A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: How do you know if you Azotobacter bacteria isolation was successful? Could you please list all…

A: Azotobacter is a genus of free-living, nitrogen-fixing bacteria that are found in soil and water.…

Q: Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus…

A: Answer.) :- To calculate the required cell density for the new culture, you can use the…

Q: If possible please answer all questions based on the gel electrophoresis: 1. What is PCR product…

A: Answer: 1] PCR product size:                               PCR ~ Polymerase Chain Reaction…

Q: Complete the following statement about conjugation. Bacterial confirmation is the equivalent of…

A: Bacterial conjugation is the process of transfer of genetic material from one bacterium to another…

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: 10) With the disk test, what specifically are you looking for to know if a bacteria is susceptible…

A: The disk test is done to find which bacteria is susceptible to which antibiotic. This test is also…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific…

A: Lambda phage exhibits a lytic or lysogenic cycle depending on the expression of certain genes.

Q: Explain why O- is considered a universal donor and why AB+ is considered a universal recipient.

A: O negative is universal donar as it has no antigen present on its surface therefore it can't elicit…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."