Look at the amino acid composition of a protein given in the table and click on the area in the 2D gel electrophoresis where you may expect the corresponding band.Amino acid compositionADFGKLMPRVYTOTAL:1621214929173113211111013164Download Image..pH7pH 9pH3200,000116,25097,40066,20045,00031,00021,50014,400| ||| | |||

the isoelectric point of an amino acid is the pH at which the net electric charge of that amino acid…

Answered: Look at the amino acid composition of a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You have a stock solution of protein that you would like to dilute. How much stock protein do you need to add to 800 µL of buffer to make a 1:5 dilution? 200ul 500ul 100ml 100ul
Please don't provide handwriting solution
Özcan MM. and his colleagues tried to obtain 3 different fatty acids from moringa pelegrina nucleus with cold pressing and soxhlet extraction by two different methods. The data is as follows; Fatty acids Cold pressing method Moringa pelegrina nucleus Soxhlet extraction method Moringa pelegrina nucleus a-tocopherol(mg/kg) 137.89 ± 2.89 y-tocopherol(mg/kg) 63.68+0.87 8-tocopherol(mg/kg) 151.67+1.89 71.57+1.06 61.49+0.76 63.58+0,97 Accordingly, 3 different fatty acids obtained by 2 different methods used; a) Calculate their efficiency. b) By which method has more efficiency been achieved and explain with the reasons?
Please calculate and plot the charge on a peptide with the sequence of NEYK over the pH range from 1 to 13. Please use the pKa values from the table. You may calculate the charge at 1 pH unit intervals.
Hand draw the expected gel result from the graphical data of three extracted RNA samples (in Figure 1a, 1b, & 1c) in Nanodrop spectrophotometer. The 3 graphical data should be in 1 agarose gel Provide complete gel label descriptions (ladder, lanes, etc.).
An influx of positive sodium ions eventually causes closing of the inactivation gates of voltage regulated sodium channels, stopping the influx of positive sodium ions, and thus the final stages of an action potential are regulated by _____ feedback.
A 100 mL of 0.1 M amino acid at pH 1.0, whose pka for the carboxyl group is less than the pKa of the R-group, was titrated with NaOH solution. The pH was monitored, and the results were plotted on a graph, as shown below. The key points in the titration are designated A to G. 12 10 8 pH 6 4 2 0 A B 0.5 с D E F 1.5 2 2.5 Equivalents of OH 1. What is the possible identity of the amino acid? [Select] 2. What is the isoelectric point of the amino acid? [Select] 3. What is the pka corresponding to the deprotonation of the alpha-amino group? [Select] 4. Region/ point where the amino acid is predominantly present as a (-2)-charged species. [Select] 5. The effective buffering range for the amino acid in the basic region. [Select] G 3
A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the proteins shown below. If these proteins are collected into separate fractions in between the void volume and total volume, in which order will they elute? Indicate if any of the proteins are found in the void volume or total volume fractions. Protein Z - 3330Da Protein Y - 13kDa Protein X - 1.3kDa I. Total volume fraction II. Third protein fraction III. Second protein fraction IV. First protein fraction V. Void volume fraction
The figure below shows the structures of the blood group antigens O, A and B.List all nucleotide sugars and glycosyltransferase enzymes that are needed to construct blood group antigen A.
phpfattempi-101408mid=10000 page # Which of the diagrams below best represents the structure of an oligosaccharide? Select one: us page a. b. O C. d. glycerol sugar amino acid fatty acid fatty acid fatty acid 111 nucleotide nucleotide nucleotide sugar amino acid 100 nucleotide nucleotide nucleotide sugar amino acid amino ac Next page
5/ n SO a- 6.46 The following data show the binding of Mg2+ ions with a protein containing n equivalent sites: [Mg2+ ] total/μM [Mg2+ ]free/μM 108 Com 35 180 65 288 501 115 248 752 446 Apply the Scatchard plot to determine n and Ka. The protein concentration is 98 µM.
Mass spectrometry and X‑ray diffraction are common biochemical techniques for characterizing proteins. Classify each statement based on whether it applies to mass spectrometry, X‑ray diffraction, or both techniques.

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