Table 1 shows the molar properties of bases in the nucleic acid from nine different living organisms. Nucleic acid Percentage (%) A+T G+C Adenine Thymine Cytosine Guanine 24.9 Escherichia coli 26.0 23.9 25.2 1.00 (K-12) Diplococcus 29.8 31.6 20.5 18.0 1.59 pneumonia Mycobacterium 15.1 14.6 34.9 35.4 0.42 tuberculosis Yeast 31.3 32.9 18.7 17.1 1.79 Paracentrotus 32.8 32.1 17.7 18.4 1.80 lividus Herring 27.8 27.5 22.2 22.6 1.23 28.6 28.4 21.4 21.5 1.33 Rat Human Species X 30.7 31.2 19.3 18.8 1.62 30.9 29.4 ? ? ? (i) What are the percentages of Guanine and Cytosine in Species X? (ii) Which organism has a higher melting point for its DNA structure compared to species X?

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i and ii

Table 1 shows the molar properties of bases in the nucleic acid from nine different living
organisms.
Nucleic acid
Percentage (%)
A+T
G+C
Adenine
Thymine
Guanine
Cytosine
Escherichia coli
26.0
23.9
24.9
25.2
1.00
(K-12)
Diplococcus
29.8
31.6
20.5
18.0
1.59
pneumonia
Mycobacterium 15.1
14.6
34.9
35.4
0.42
tuberculosis
Yeast
31.3
32.9
18.7
17.1
1.79
Paracentrotus
32.8
32.1
17.7
18.4
1.80
lividus
Herring
27.8
27.5
22.2
22.6
1.23
Rat
28.6
28.4
21.4
21.5
1.33
Human
30.7
31.2
19.3
18.8
1.62
Species X
30.9
29.4
?
?
?
(i)
What are the percentages of Guanine and Cytosine in Species X?
(ii)
Which organism has a higher melting point for its DNA structure compared to species
X?
Explain why DNA in the presence of monovalent cations will have a high melting
temperature (Tm).
(iv)
Erwin Chargaff's research was based on the selection of DNAs from many organisms
(Table 1). If Erwin's analyses is based on only the data of Escherichia coli (K12), would
he be able to establish certain Chargaff's rule? Explain.
Transcribed Image Text:Table 1 shows the molar properties of bases in the nucleic acid from nine different living organisms. Nucleic acid Percentage (%) A+T G+C Adenine Thymine Guanine Cytosine Escherichia coli 26.0 23.9 24.9 25.2 1.00 (K-12) Diplococcus 29.8 31.6 20.5 18.0 1.59 pneumonia Mycobacterium 15.1 14.6 34.9 35.4 0.42 tuberculosis Yeast 31.3 32.9 18.7 17.1 1.79 Paracentrotus 32.8 32.1 17.7 18.4 1.80 lividus Herring 27.8 27.5 22.2 22.6 1.23 Rat 28.6 28.4 21.4 21.5 1.33 Human 30.7 31.2 19.3 18.8 1.62 Species X 30.9 29.4 ? ? ? (i) What are the percentages of Guanine and Cytosine in Species X? (ii) Which organism has a higher melting point for its DNA structure compared to species X? Explain why DNA in the presence of monovalent cations will have a high melting temperature (Tm). (iv) Erwin Chargaff's research was based on the selection of DNAs from many organisms (Table 1). If Erwin's analyses is based on only the data of Escherichia coli (K12), would he be able to establish certain Chargaff's rule? Explain.
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