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In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? O 30 bases O 90 bases 900 bases 273 bases

In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? O 30 bases O 90 bases 900 bases 273 bases

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You perform a translation experiment with mRNA and all required molecules in two identical test tubes. You add limiting amounts of the antibiotic puromycin to test tube 2 only. Puromycin is a molecule that has structural similarities to the 3' end of a charged TRNA. It can enter the ribosome and be incorporated into the growing protein. When puromycin is incorporated into the polypeptide, it stalls the ribosome and the polypeptide is released. You do not know if puromycin recognizes a specific codon or not. You examine the length of the polypeptide produced in both test tubes. In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? 30 bases 90 bases O 900 bases O 273 bases
Transcribed Image Text:You perform a translation experiment with mRNA and all required molecules in two identical test tubes. You add limiting amounts of the antibiotic puromycin to test tube 2 only. Puromycin is a molecule that has structural similarities to the 3' end of a charged TRNA. It can enter the ribosome and be incorporated into the growing protein. When puromycin is incorporated into the polypeptide, it stalls the ribosome and the polypeptide is released. You do not know if puromycin recognizes a specific codon or not. You examine the length of the polypeptide produced in both test tubes. In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? 30 bases 90 bases O 900 bases O 273 bases
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