You synthesized 3 pre-mRNAs, as shown, performed splicing in a test tube, and monitored the splicing reaction on a gel by autoradiography. What can you conclude from this data? Select all that apply. You may select multiple options. These pre-mRNAs were incubated with a nuclear extract and splicing over time (0-120 min) was followed by gel electrophoresis. m7GpppG 0 30 60 120 m/Gppp 13- Gppp 13- (uncapped) 13- GpppG 0 30 60 120 O Cap affects the splicing of the second intron O None of these options O Cap affects the splicing of the first intron O Cap affects splicing of all introns O Cap has no effect on splicing exon intron 14 14- uncapped 0 30 60 120 min 15 Fig. 15.29 13 14 13 14 15 () 15 13 14 15 (0) 13 14 15 (4)
You synthesized 3 pre-mRNAs, as shown, performed splicing in a test tube, and monitored the splicing reaction on a gel by autoradiography. What can you conclude from this data? Select all that apply. You may select multiple options. These pre-mRNAs were incubated with a nuclear extract and splicing over time (0-120 min) was followed by gel electrophoresis. m7GpppG 0 30 60 120 m/Gppp 13- Gppp 13- (uncapped) 13- GpppG 0 30 60 120 O Cap affects the splicing of the second intron O None of these options O Cap affects the splicing of the first intron O Cap affects splicing of all introns O Cap has no effect on splicing exon intron 14 14- uncapped 0 30 60 120 min 15 Fig. 15.29 13 14 13 14 15 () 15 13 14 15 (0) 13 14 15 (4)
Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:You synthesized 3 pre-mRNAs, as shown, performed splicing in a test tube, and monitored the splicing reaction on a gel by autoradiography. What can you conclude from
this data? Select all that apply. You may select multiple options.
These pre-mRNAs were
incubated with a nuclear
extract and splicing over time
(0-120 min) was followed by
gel electrophoresis.
m7GpppG
0 30 60 120
O None of these options
m'Gppp 13-
Gppp 13-
(uncapped)13-
GpppG
0 30 60 120
O Cap affects the splicing of the second intron
O Cap affects the splicing of the first intron
Cap affects splicing of all introns
Cap has no effect on splicing
exon
intron
uncapped
0 30 60 120 min
Fig. 15.29
13 14
13 14 15 (0)
15
13 14
13 14 15 (4)
15 (0)
Fig. 15.30
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